Verified Reactivity：Human, Cynomolgus, Rhesus

Reported Reactivity：African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey

Antibody Type：Monoclonal

Host Species：Mouse

Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation：The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.

Concentration：Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application：FC - Quality tested

Recommended Usage：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser：Red Laser (633 nm)

Application Notes：Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Application References：(PubMed link indicates BioLegend citation)

1. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.

2. Campanelli R, et al. 2002. Intl. Immunol. 14:39.

3. Evans RL, et al. 1981. Immunol. 78:544.

4. Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.

5. Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed

6. Carbone A, et al. 1999. Blood 93:2319. (IHC-F)

7. Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)

8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

9. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed

10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Chheda ZS, et al. 2018. J Exp Med. 9:19490. PubMed

2. Wang F, et al. 2018. Oncogenesis. 7:41. PubMed

3. Su S et al. 2018. Cell. 175(2):442-457 . PubMed

4. Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed

5. Li B, et al. 2019. Oncogenesis. 8:17. PubMed

6. Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed

7. Forsberg EMV, et al. 2019. Cancer Res. 79:899. PubMed

8. Zhao L, et al. 2020. Mol Ther Oncolytics. 16:262. PubMed

9. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed

10. Oliveira A, et al. 2013. Immunology. 140:47. PubMed

11. Leitner J, et al. 2015. J Immunol. 195: 477 - 487. PubMed

12. Javed A, et al. 2015. PLoS One. 10: 0142086. PubMed

See More

RRID

AB_2075390 (BioLegend Cat. No. 344721)
AB_2075388 (BioLegend Cat. No. 344722)