Isotype Control:PE Mouse IgG1, κ Isotype Ctrl (FC)
Verified Reactivity:Human
Antibody Type:Monoclonal
Host Species:Mouse
Immunogen:Human LAG-3 transfected cells.
Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation:The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration:Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:FC - Quality tested
Recommended Usage:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser:Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes:The staining of clone 11C3C65 cannot be blocked by clone 7H2C65, which is another anti-human CD223 (LAG-3) antibody.
Product Citations
Zuazo M, et al. 2019. EMBO Mol Med. 11(7). PubMed
Yeo L, et al. 2018. J Clin Invest. 128:3460. PubMed
Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
RRID
AB_2629591 (BioLegend Cat. No. 369305)
AB_2629592 (BioLegend Cat. No. 369306)