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Isotype Control：PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl Verified Reactivity：Human Antibody Type：Monoclonal Host Species：Mouse Immunogen：Human Tim-3 fusion protein Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) Preparation：The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions. Concentration：Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application：FC - Quality tested Recommended Usage：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Excitation Laser：Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes：Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3. Additional Product Notes：BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions. Application References：(PubMed link indicates BioLegend citation) Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim) Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block) Klibi J, et al 2009. Blood 113:1957. (FC, Block)
Product Citations Jespersen H, et al. 2017. Nat Commun. 10.1038/s41467-017-00786-z. PubMed Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed Zhou R, et al. 2020. Immunity. S1074-7613(20)30333-2.. PubMed AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed Fisher J, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.03.002. PubMed Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed Riberdy JM, et al. 2020. Mol Ther Methods Clin Dev. 1.146527778. PubMed Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
RRID AB_2561719 (BioLegend Cat. No. 345013)
AB_2561720 (BioLegend Cat. No. 345014)
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