Isotype Control:PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl
Verified Reactivity:Human
Antibody Type:Monoclonal
Host Species:Mouse
Immunogen:YT cell line.
Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation:The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration:Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:FC - Quality tested
Recommended Usage:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser:Blue Laser (488 nm)
Application Notes:Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.
We recommend using our Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) (Cat. No. 929501).
Additional Product Notes:BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References:(PubMed link indicates BioLegend citation)
Roncador G, et al. 2007. Haematologica 92:1059.
Nam-Cha SH, et al. 2008. Am. J. Surg. Pathol. 32:1252.
Rodriguez-Pinilla SM, et al. 2009. Am. J. Surg. Pathol. 33:81.
Product Citations
Park JA, et al. 2020. J Hematol Oncol. 0.661111111. PubMed
RRID
AB_2566679 (BioLegend Cat. No. 367409)
AB_2566680 (BioLegend Cat. No. 367410)