Isotype Control：PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl
Verified Reactivity：Human
Antibody Type：Monoclonal
Host Species：Mouse
Immunogen：Extracellular domain of human CTLA-4 and human IgG1 Fc fusion protein
Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation：The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration：Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application：ICFC - Quality tested
Recommended Usage：Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser：Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes：ELISA Detection: The biotinylated L3D10 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified A3.6B10.G1 antibody (Cat. No. 525401) as the capture antibody and recombinant human CTLA-4 (Cat. No. 591909) as the standard.
Flow Cytometry: The fluorochrome-labeled L3D10 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify CTLA-4-producing cells within mixed cell populations.
Note: For testing human soluble CTLA-4 in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Soluble CTLA-4 ELISA Kit with Pre-coated Plates (Cat. No. 437407) are specially developed and recommended.
Additional reported applications (for the relevant formats) include: Blocking of CTLA-4/B7-1 interaction and blocking of CTLA-4-mediated inhibitory function to promote T cell expansion1,2.
This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of frozen or paraffin-embedded tissue sections (IHC-F or IHC-P).
Additional Product Notes：BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References：
(PubMed link indicates BioLegend citation)
May K, et al. 2005. Blood 105:1114. (Block)
Lute K, et al. 2005. Blood 106:3127. (Block)
Product Citations
Sander F, et al. 2017. Cancer Immunol Immunother. 10.1007/s00262-017-2040-9. PubMed
Zheng Z, et al. 2017. PLoS One. 10.1371/journal.pone.0184127. PubMed
Li X, et al. 2019. Front Oncol. 1.115277778. PubMed
Wang A, et al. 2020. Front Immunol. 1.213194444. PubMed
RY H, et al. 2016. Oncoimmunology. 6:e1249561. PubMed
Luoma AM, et al. 2020. Cell. 182(3):655-671.e22. PubMed
Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
RRID
AB_2563097 (BioLegend Cat. No. 349913)
AB_2563098 (BioLegend Cat. No. 349914)
