Isotype Control:PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl
Verified Reactivity:Human
Reported Reactivity:Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type:Monoclonal
Host Species:Mouse
Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation:The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration:Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:FC - Quality tested
Recommended Usage:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser:Blue Laser (488 nm)
Application Notes:Additional reported applications include: immunocytochemistry3.
Additional Product Notes:BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References:
(PubMed link indicates BioLegend citation)
Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations
Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed
Chen EW, et al. 2019. Front Immunol. 10:1718. PubMed
RRID
AB_2125479 (BioLegend Cat. No. 302625)
AB_2125478 (BioLegend Cat. No. 302626)