Isotype Control ：PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl

• Verified Reactivity：Human, Cynomolgus, Rhesus

• Reported Reactivity：African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Pigtailed Macaque, Sooty Mangabey

• Antibody Type：Monoclonal

• Host Species：Mouse

• Immunogen：CD95 transfected L cells

• Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

• Preparation：The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.

• Concentration：Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

• Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

• Application：FC - Quality tested

• Recommended Usage：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

• Excitation Laser：Blue Laser (488 nm)

• Green Laser (532 nm)/Yellow-Green Laser (561 nm)
  

• Application Notes：The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656).

• Note: EOS9.1 antibody can induce apoptosis without cross-linking.

• Additional Product Notes：BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

• Application References：(PubMed link indicates BioLegend citation)

• 
  

1. Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York.

2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.

3. Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop)

4. Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC)

5. Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC)

6. Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed

7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

8. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.

9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)

10. Dixit N, et al. 2012. J. Immunol. 189:5954. PubMed

11. 
    

• Product Citations：

1. Fitzsimmons L, et al. 2018. Cell Death Differ. 25:241. PubMed

2. Felices M, et al. 2018. JCI Insight. 3. PubMed

3. Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed

4. Siddiqui I et al. 2019. Immunity. 50(1):195-211 . PubMed

5. Cosgrove C, et al. 2013. Blood. 121:951. PubMed

6. Marco M, et al. 2013. Transpl Immunol. 29:88. PubMed

7. Lewis M, et al. 2015. Am J Hum Genet. 96:221. PubMed

8. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed

9. Piepenbrink M, et al. 2016. PLoS One. 11: 0158641. PubMed

10. Berry N, et al. 2016. PLoS Pathog. 12:e1006083. PubMed

11. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed

12. Pais Ferreira D, et al. 2020. Immunity. 53(5):985-1000.e11. PubMed

• RRID

• AB_2100370 (BioLegend Cat. No. 305621)
  AB_2100369 (BioLegend Cat. No. 305622)