• Isotype Control： FITC Mouse IgG1, κ Isotype Ctrl

• Verified Reactivity：Human

• Reported Reactivity：Chimpanzee

• Antibody Type：Monoclonal

• Host Species：Mouse

• Formulation：µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

• Preparation：The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

• Concentration：µg sizes: 0.5 mg/mL  test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

• Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

• Application：FC - Quality tested

• Recommended Usage：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

• Excitation Laser：Blue Laser (488 nm)

• Application Notes：Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

• Application References：(PubMed link indicates BioLegend citation)

   1.Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)

  2.Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)

  3.Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)

  4.Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)

  5.Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)

  6.Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)

  7.Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)

  8.Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed

  9.Van Dongen JJM, et al. 1988. Blood 71:603. (WB)

  10.Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

  11.Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)

  12.Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed

• Product Citations

• See More

1. Engelmann C, et al. 2017. J Transl Med. 10.1186/s12967-017-1288-3. PubMed

2. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed

3. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed

4. Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed

5. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed

6. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed

7. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed

8. Johnson DC, et al. 2018. Nat Med. 24:1151. PubMed

9. Ray RM, et al. 2019. Sci Rep. 9:4334. PubMed

10. Chen L, et al. 2018. Immunology. 155:123. PubMed

11. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed

12. Coufal S, et al. 2019. Cells. 8. PubMed

• RRID

• AB_314059 (BioLegend Cat. No. 300405)
  AB_2564148 (BioLegend Cat. No. 300452)
  AB_314060 (BioLegend Cat. No. 300406)
  AB_2562046 (BioLegend Cat. No. 300440)