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Cat. Number
芘 亚磷酰胺 dU Pyrene phosphoramidite dU
Chemical Name
芘 亚磷酰胺 dU Pyrene phosphoramidite dU
Mol. Formula
C57H55N4O8P
Mol. Weight
955.04
Qty 1
250mg
Qty 2
1g
Appearance
yellowish foam
Solubility
good in dichloromethane and acetonitrile
Storage condition
-20°C
References

Pyrene is polycyclic aromatic hydrocarbon which is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene.

With this phosphoramidite, pyrene can be introduced into DNA or RNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows to attach pyrene fragment to 5', internal, or 3' position (using universal support).

This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.