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Cat. Number
Long trebler 亚磷酰胺 Long trebler phosphoramidite
Chemical Name
Long trebler 亚磷酰胺 Long trebler phosphoramidite
Mol. Formula
C89H107N2O15P
Mol. Weight
1475.78
Qty 1
250mg
Qty 2
500mg
Appearance
viscous yellowish oil
Solubility
soluble in acetonitrile, dichloromethane
Storage condition
-20°C
References

Long trebler phosphoramidite is a branching reagent for oligonucleotide synthesis allowing to synthesize branched DNA structures using standard DNA synthesizer.

use of trebler amidite to synthesize branched DNA

After the condensation of the trebler, three DNA branches begin to grow simultaneously with each step of the synthesis. Deblock of this construct gives rise to DNA containing branching point. One arm (stem) is attached to branch point with its 5'-end, and other arms (branches) are attached via 3'-end. Reverse amidites can be used to prepare constructs with different branch orientations. Repetitive condensations of trebler results in formation of DNA dendrimers.

Trebler amidite can be used to attach several modifier amidites to 5'-end of oligonucleotide - for example, three biotin residues can be attached at once.

This amidite does not require any special handling. Recommended coupling time is 5 minutes. No changes to deblock conditions are required.