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Cat. Number
炔烃亚磷酰胺,5' 端 Alkyne Phosphoramidite, 5'-terminal
Mol. Formula
C21H36N3O3P
Mol. Weight
409.50
Qty 1
250mg
Qty 2
1g
Appearance
colorless solid
Solubility
good in acetonitrile and dichloromethane
Storage condition
-20°C
References

Phosphoramidite for the synthesis of oligonucleotides with 5'-terminal alkyne for Click Chemistry.

This alkyne amidite has several advantages over 5’-hexynyl phosphoramidite, 5'-butynyl-CEP, and other 5'-terminal alkyne phosphoramidites. First, it is solid compound which is easier to handle and dispense. And due to its structure, it is also more stable in solution, and has longer shelf life

Diluent for this phosphoramidite is acetonitrile, 5 min coupling time is recommended. Because this amidite does not contain 5'-terminal DMT group, no 5'-deprotection needed. Oligonucleotides should be deblocked under standard conditions, and purified by PAGE, or ion exchange HPLC.

Oligonucleotides with this modification are ideal for the use in Click Chemistry (see our recommendedprotocol).

Oligo synthesis details

Coupling conditions: Standard coupling, identical to normal nucleobases
Deprotection conditions: No deprotection required. Compatible with standard deprotection reagents.

Product citations

  1. Aparin, I.O.; Farzan, V.M.; Veselova, O.A.; Chistov, A.A.; Podkolzin, A.T.; Ustinov, A.V.; Shipulin, G.A.; Formanovsky, A.A.; Korshun, V.A.; Zatsepin, T.S. 1-Phenylethynylpyrene (PEPy) as a novel blue-emitting dye for qPCR assay. Analyst2016, 141, 1331–1338. doi: 10.1039/c5an01767j
  2. Astakhova, I.K.; Santhosh Kumar, T.; Campbell, M.A.; Ustinov, A.V.; Korshun, V.A.; Wengel, J. Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene-perylene 2'-alpha-L-amino-LNA FRET pairs. Chemical Communications2013, 49(5), 511-511. doi: 10.1039/c2cc37547h