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Cat. Number
SYBR Green I凝胶染色溶液 dsGreen Gel Staining Solution, 10000×
Chemical Name
SYBR Green I凝胶染色溶液 dsGreen Gel Staining Solution, 10000×
Qty 1
1ml
Appearance
orange solution
Storage condition
-20°C
References

dsGreen, an equivalent of SYBR® Green I is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels. A qPCR grade reagent is also available.

Unlike ethidium bromide, dsGreen is highly selective towards double stranded DNA, much less harmful, and offers better sensitivity.

Comparison between ethidium bromide and dsGreen

Feature Ethidium Bromide dsGreen
Fluorescence Red (615 nm) Green (524 nm)
Excitation maximum 302 nm 454 nm
Excitation light source UV only Blue light or UV
Sensitivity 2 ng / band (dsDNA)
100 ng / band (RNA)
0.08 ng / band (dsDNA)
1–2 ng / band (oligonucleotides)
Health hazard High Low

Note: Fluorescence properties of SYBR Green I bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.

Spectral properties

Excitation maximum, nm: 454
ε, L⋅mol−1⋅cm−1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0.8

Product citations

  1. Zietzer, A.; Buschmann, E.E.; Janke, D.; Li, L.; Brix, M.; Meyborg, H.; Stawowy, P.; Jungk, C.; Buschmann, I.; Hillmeister, P. Acute Physical Exercise and Long-Term Individual Shear Rate Therapy Increase Telomerase Activity in Human Peripheral Blood Mononuclear Cells. Acta Physiologica, in press. doi:10.1111/apha.12820
  2. Diaz-Romero, J.; Kürsener, S.; Kohl, S.; Nesic, D. S100B+A1 CELISA: A Novel Potency Assay and Screening Tool for Redifferentiation Stimuli of Human Articular Chondrocytes. Journal of Cellular Physiology2017, 232(6), 1559–1570. doi: 10.1002/jcp.25682
  3. Richter-Heitmann, T.; Eickhorst, T.; Knauth, S.; Friedrich, M.W.; Schmidt, H. Evaluation of Strategies to Separate Root-Associated Microbial Communities: A Crucial Choice in Rhizobiome Research. Frontiers in Microbiology2016, 7, 773. doi: 10.3389/fmicb.2016.00773
  4. Jain, D.; Siede, W. Rad5 Template Switch Pathway of DNA Damage Tolerance Determines Synergism between Cisplatin and NSC109268 in Saccharomyces cerevisiaePLoS ONE2013, 8(10), e77666. doi:10.1371/journal.pone.0077666