SYBR Green I凝胶染色溶液 dsGreen Gel Staining Solution, 10000×_上海惠诚生物生物试剂,标准品,仪器设备,耗材,一站式服务

dsGreen, an equivalent of SYBR® Green I is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels. A qPCR grade reagent is also available.

Unlike ethidium bromide, dsGreen is highly selective towards double stranded DNA, much less harmful, and offers better sensitivity.

Comparison between ethidium bromide and dsGreen

Feature	Ethidium Bromide	dsGreen
Fluorescence	Red (615 nm)	Green (524 nm)
Excitation maximum	302 nm	454 nm
Excitation light source	UV only	Blue light or UV
Sensitivity	2 ng / band (dsDNA) 100 ng / band (RNA)	0.08 ng / band (dsDNA) 1–2 ng / band (oligonucleotides)
Health hazard	High	Low

Recommended protocol

DNA Staining in Gels with dsGreen or SYBR Green I

Note: Fluorescence properties of SYBR Green I bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.

Spectral properties

Excitation maximum, nm:	454
ε, L⋅mol−1⋅cm−1:	73000
Emission maximum, nm:	524
Fluorescence quantum yield:	0.8

Product citations

Zietzer, A.; Buschmann, E.E.; Janke, D.; Li, L.; Brix, M.; Meyborg, H.; Stawowy, P.; Jungk, C.; Buschmann, I.; Hillmeister, P. Acute Physical Exercise and Long-Term Individual Shear Rate Therapy Increase Telomerase Activity in Human Peripheral Blood Mononuclear Cells. Acta Physiologica, in press. doi:10.1111/apha.12820
Diaz-Romero, J.; Kürsener, S.; Kohl, S.; Nesic, D. S100B+A1 CELISA: A Novel Potency Assay and Screening Tool for Redifferentiation Stimuli of Human Articular Chondrocytes. Journal of Cellular Physiology, 2017, 232(6), 1559–1570. doi: 10.1002/jcp.25682
Richter-Heitmann, T.; Eickhorst, T.; Knauth, S.; Friedrich, M.W.; Schmidt, H. Evaluation of Strategies to Separate Root-Associated Microbial Communities: A Crucial Choice in Rhizobiome Research. Frontiers in Microbiology, 2016, 7, 773. doi: 10.3389/fmicb.2016.00773
Jain, D.; Siede, W. Rad5 Template Switch Pathway of DNA Damage Tolerance Determines Synergism between Cisplatin and NSC109268 in Saccharomyces cerevisiae. PLoS ONE, 2013, 8(10), e77666. doi:10.1371/journal.pone.0077666

下一个：SYBR Green I染料，用于实时PCR dsGreen for Real-Time PCR, 100× 上一个：ROX 参考染料，用于qPCR ROX reference dye for qPCR

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