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Cat. Number
Cy5 羧酸 Cyanine5 carboxylic acid
Chemical Name
Cy5 羧酸 Cyanine5 carboxylic acid
Mol. Formula
C32H39ClN2O2
Mol. Weight
519.12
Qty 1
5mg
Qty 2
25mg
Appearance
dark blue powder
Solubility
soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Storage condition
-20°C
References

Non-activated carboxylic acid, an analog of Cy5® free carboxylic acid. Contains Cyanine5 fluorophore.

This dye has limited water solubility, but can be dissolved in mixtures of water with organic phase (DMF, DMSO, alcohols) to obtain useful concentrations of the material in solution. Water-soluble version is available.

This molecule can be considered non-reactive dye for the use in control samples, and for instrument calibration. For coupling with amines and protein labeling, consider using Cyanine5 NHS ester, or water-soluble sulfo-Cyanine5 NHS ester.

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2

Product citations

  1. Kwok, S.J.J.; Choi, M.; Bhayana, B.; Zhang, X.; Ran, C.; Yun, S.-H. Two-photon excited photoconversion of cyanine-based dyes. Scientific Reports2016, 6, 23866. doi: 10.1038/srep23866

  2. Gupta, A.; Verma, N.C.; Khan, S.; Tiwari, S.; Chaudhary, A.; Nandi, C.K. Paper strip based and live cell ultrasensitive lead sensor using carbon dots synthesized from biological media. Sensors and Actuators B: Chemical2016, 232, 107–114. doi: 10.1016/j.snb.2016.03.110

  3. Deyle, K.M.; Farrow, B.; Hee, Y.Q.; Work, J.; Wong, M.; Lai, B.; Umeda, A.; Millward, S.W.; Nag, A.; Das, S.; Heath, J.R. A protein-targeting strategy used to develop a selective inhibitor of the E17K point mutation in the PH domain of Akt1. Nature Chemistry2015, 7, 455–462. doi: 10.1038/nchem.2223

  4. Rand, M.D.; Montgomery, S.L.; Prince, L.; Vorojeikina, D. Developmental Toxicity Assays Using the Drosophila Model. Current Protocols in Toxicology2014, 1.12.1-1.12.20. doi:10.1002/0471140856.tx0112s59

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