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Cat. Number

Cy5 马来酰亚胺 Cyanine5 maleimide

Chemical Name

Cy5 马来酰亚胺 Cyanine5 maleimide

Mol. Formula

C38H45ClN4O3

Mol. Weight

641.24

Qty 1

5mg

Qty 2

25mg

Appearance

dark blue powder

Solubility

soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water

Storage condition

-20°C

References

Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.

Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.

For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.

Spectral properties

Excitation maximum, nm:	646
ε, L⋅mol−1⋅cm−1:	250000
Emission maximum, nm:	662
Fluorescence quantum yield:	0.2

Product citations

Chen, L.; Weinmeister, R.; Kralovicova, J.; Eperon, L.P.; Vorechovsky, I.; Hudson, A.J.; Eperon, I.C.Stoichiometries of U2AF35, U2AF65 and snRNP reveal new early spliceosome assembly pathways. Nucleic Acids Research, 2017, 45(4), 2051–2067. doi: 10.1093/nar/gkw860
Hinde, E.; Thammasiraphop, K.; Duong, H.T.T.; Yeow, J.; Karagoz, B.; Boyer, C.; Gooding, J.J.; Gaus, K.Pair correlation microscopy reveals the role of nanoparticle shape in intracellular transport and site of drug release. Nature Nanotechnology, 2017, 12(1), 81–89. doi: 10.1038/nnano.2016.160
Sahle, F.F.; Giulbudagian, M.; Bergueiro, J.; Lademann, J.; Calderón, M. Dendritic polyglycerol and N-isopropylacrylamide based thermoresponsive nanogels as smart carriers for controlled delivery of drugs through the hair follicle. Nanoscale, 2017, 9(1), 172–182. doi: 10.1039/c6nr06435c
Jackson, A.; Jani, S.; Sala, C.D.; Soler-Bistué, A.J.C.; Zorreguieta, A.; Tolmasky, M.E. Assessment of configurations and chemistries of bridged nucleic acids-containing oligomers as external guide sequences: a methodology for inhibition of expression of antibiotic resistance genes. Biology Methods and Protocols,2016, 1(1), bpw001. doi: 10.1093/biomethods/bpw001

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