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Cat. Number

Cy3 马来酰亚胺 Cyanine3 maleimide

Chemical Name

Cy3 马来酰亚胺 Cyanine3 maleimide

Mol. Formula

C36H43ClN4O3

Mol. Weight

615.20

Qty 1

5mg

Qty 2

25mg

Appearance

red powder

Solubility

soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water

Storage condition

-20°C

References

Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).

Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.

Labeling with Cyanine3 maleimide is selective, and efficient.

We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.

Spectral properties

Excitation maximum, nm:	555
ε, L⋅mol−1⋅cm−1:	150000
Emission maximum, nm:	570
Fluorescence quantum yield:	0.31

Product citations

Wang, H.; Wu, J.; Xu, L.; Xie, K.; Chen, C.; Dong, Y. Albumin Nanoparticle Encapsulation of Potent Cytotoxic Therapeutics Shows Sustained Drug Release and Alleviates Cancer Drug Toxicity. Chemical Communications, 2017, 53(17), 2618–2621. doi: 10.1039/c6cc08978j
Xue, C.; Whitis, N.R.; Sashital, D.G. Conformational Control of Cascade Interference and Priming Activities in CRISPR Immunity. Molecular Cell, 2016, 64(4), 826–834. doi: 10.1016/j.molcel.2016.09.033
Chiu, H.-Y.; Deng, W.; Engelke, H.; Helma, J.; Leonhardt, H.; Bein, T. Intracellular chromobody delivery by mesoporous silica nanoparticles for antigen targeting and visualization in real time. Scientific Reports,2016, 6, 25019. doi: 10.1038/srep25019
Gaitzsch, J.; Delahaye, M.; Poma, A.; Du Prez, F.; Battaglia, G. Comparison of metal free polymer-dye conjugation strategies in protic solvents. Polymer Chemistry, 2016, 7(17), 3046–3055;. doi:10.1039/c6py00518g

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