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Cat. Number
Cyanine3 azide
Chemical Name
Cyanine3 azide
Mol. Formula
C33H43ClN6O
Mol. Weight
575.19
Qty 1
5mg
Qty 2
10mg
Appearance
red powder / solution
Storage condition
-20°C
References

Cyanine3 dye azide for Click Chemistry, an analog of Cy3® azide. Cy3® is one of the most broadly used fluorophores which can be detected by various fluorometers, imagers, and microscopes. Due to inherently high extinction coefficient, Cyanine3 is also easily detected by naked eye on gels, and in solution. This is non-sulfonated dye which requires organic co-solvent (DMF, DMSO, or other) for efficient labeling in water. Water-soluble version of this reagent is also available.

Product is available both as solid compound, and as 10 mM solution in DMSO which is ready to use in our recommended protocol.

Cyanine3 fluorescent properties are identical to Cy3®, and similar to Alexa Fluor 546, and DyLight 549.

 

Product citations

  1. Tsai, C.-Y.; Li, C.-W.; Li, J.-R.; Jang, B.-H.; Chen, S.-H. Steroid Probes Conjugated with Protein-Protected Gold Nanocluster: Specific and Rapid Fluorescence Imaging of Steroid Receptors in Target Cells. Journal of Fluorescence2016, 26(4), 1239–1248. doi: 10.1007/s10895-016-1811-6

  2. Park, H.; Koo, J.Y.; Srikanth, Y.V.V.; Oh, S.; Lee, J.; Park, J.; Park, S.B. Nonspecific protein labeling of photoaffinity linkers correlates with their molecular shapes in living cells. Chemical Communications2016, 52(34), 5828–5831. doi: 10.1039/c6cc01426g

  3. Zilio, C.;Bernardi, A.; Palmioli, A.; Salina, M.; Tagliabue, G.; Buscaglia, M.; Consonni, R.; Chiari, M. New "clickable" polymeric coating for glycan microarrays. Sensors and Actuators B: Chemical2015, 215, 412–420. doi: 10.1016/j.snb.2015.03.079

  4. Hong, T.N.; van der Hoorn, R.A.L. DIGE-ABPP by Click Chemistry: Pairwise Comparison of Serine Hydrolase Activities from the Apoplast of Infected Plants. Plant-Pathogen Interactions (Methods in Molecular Biology)2014, 1127, 183-194. doi: 10.1007/978-1-62703-986-4_15

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