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Cat. Number
Coumarin 343 X NHS ester
Chemical Name
Coumarin 343 X NHS ester
Mol. Formula
C26H29N3O7
Mol. Weight
495.52
Qty 1
5mg
Qty 2
25mg
Appearance
yellow solid
Storage condition
-20°C
References

Blue emitting Coumarin 343 dye can form a FRET pair with fluorescein (FAM). An amine reactive form, activated NHS ester.

This amine-reactive NHS ester contains an aminohexanoyl linker between the fluorophore, and the reactive group. This linker provides better solubility and spatial separation between the fluorophore, and the target molecule being labeled.

This reagent can be used to design FRET based assays with FAM as acceptor, and to construct systems which harvest blue light energy.

General properties

Appearance: yellow solid
Molecular weight: 495.52
Molecular formula: C26H29N3O7
Solubility: good in DCM, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:Download

Spectral properties

Excitation maximum, nm: 437
ε, L⋅mol−1⋅cm−1: 44000
Emission maximum, nm: 477
Fluorescence quantum yield: 0.63
CF260: 0.29
CF280: 0.24
下一个:Cyanine3 NHS ester上一个:BDP TR NHS ester
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