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Cat. Number
HM0019
Chemical Name
己糖激酶
CAS Number
9001-51-8
Mol. Weight
~ 54 kDa (monomer);~110 kDa (dimer)
Appearance
白色粉末。
Application Notes
BR ,生化研究。
Synonym
HK;Hexokinase from Saccharomyces cerevisiae;ATP:D-Hexose-6-phosphotransferase;Hexokinase from yeast 其他名称:己糖磷酸激酶
Storage condition
-20℃
References

活力:~ 100 units/mg protein

活力定义:One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25℃
Optimal pH:7.5~9.0

性状:酿酒酵母提取。催化剂(Activators):Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds;抑制剂(Inhibitors):sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
用途:为催化己糖6位上由ATP使之产生磷酸化反应的酶。对α-D葡萄糖的亲和性很高(Km=1×10-5M),对其他的己糖也可进行磷酸化。此反应的平衡向右偏移。△G°’=4.0千卡。该酶可被Mg2 、K 所活化,但可被HS试剂阻抑。

己糖激酶 HK  BR 10KU ¥2700
己糖激酶 HK  BR 2.5KU ¥1100
己糖激酶 HK  BR 1KU ¥800
己糖激酶 HK  BR 500U ¥600