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/ Products Classification 点击展开+| Cat. Number | HM0190 |
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| Chemical Name | HM0190 荧光素酶 61970-00-1 |
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| CAS Number | 61970-00-1 |
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| Appearance | 粉末。EC：1.13.12.7 |
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| Application Notes | BR,生化研究 |
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| Synonym | Luciferase firefly；Luciferase from Photinus pyralis (firefly) 荧光素酶(萤火虫)；重组型酵素 |
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| Storage condition | -20℃。保质期1年 |
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| References | product line：BioUltra Assay：≥98% (SDS-PAGE) Mol wt：mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity) Protein：10-35% E1%/280 Measuring range：≤1 femtomole ATP sensitivity, (using 0.2 μg of luciferase and suitably sensitive liquid scintillation counters or luminometers) Foreign activity：ATPase ≤5 nanomolar units/mg protein；Nucleoside diphosphokinase ≤20 nanomolar units/mg protein 活力定义：One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C 产品描述：Two contaminant, ATP-consuming activities are assayed for in this product, ATPase and nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein and less than 20 nanomolar units/mg protein, respectively. Note: Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP and 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximately 1.3 x 106 light units
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