Activity：≥10u/mg
活力定义：One Unit increases the absorbance at 260 nm by 0.200 in 30 minutes at 37℃, pH 6.5, with an RNA substrate
产品描述：Spleen exonuclease (Phosphodiesterase II) excises 3'-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique
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磷酸二酯酶Ⅱ |
Phosphodiesterase II from bovine spleen BR |
10U | ¥1400 |
