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/ Products Classification 点击展开+| Cat. Number | HM0198 |
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| Chemical Name | HM0198 蛋白酶S 66676-43-5 |
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| CAS Number | 66676-43-5 |
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| Appearance | 粉末 |
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| Application Notes | BR,生化研究 |
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| Synonym | Protease S Aureus；Endoproteinase Glu-C from Staphylococcus aureus V8；V8 Protease |
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| Storage condition | 2～8℃ |
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| References | 来源：Staph aureus V8 Activity：≥500units/mg 活力定义：One Unit causes a change of 0.001 A280 nm per minute at 37℃, pH 7.8 using casein as the substrate 产品描述：Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).
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