| References |
| Synonyms |
- Peroxisome Proliferator-activated Receptor α Ligand Binding Domain
|
| Formulation |
A solution in 50 mM sodium phosphate, pH 7.2, containing 20% glycerol, 100 mM sodium chloride, and 1 mM DTT |
| Purity |
≥90% |
| Stability |
6 months |
| Storage |
-80°C |
| Shipping |
Dry ice
in continental US; may vary elsewhere
|
Background Reading
Kersten, S., Desvergne, B., and Wahli, W. Roles of PPARs in health and disease. Nature 405 421-424 (2000).
Berger, J., Leibowitz, M.D., Doebber, T.W., et al. Novel peroxisome proliferator-activated receptor (PPAR) γ and PPARδ ligands produce distinct biological effects. J Biol Chem 274 6718-6725 (1999).
Devchand, P.R., Keller, H., Peters, J.M., et al. The PPARα-leukotriene B4 pathway to inflammation control. Nature 384 39-43 (1996).
Lehmann, J.M., Lenhard, J.M., Oliver, B.B., et al. Peroxisome proliferator-activated receptors a and g are activated by indomethacin and other non-steroidal anti-inflammatory drugs. J Biol Chem 272 3406-3410 (1997).
Forman, B.M., Chen, J., and Evans, R.M. Hypolipidemic drugs, polyunsaturated fatty acids, and eicosanoids are ligands for peroxisome proliferator-activated receptors α and δ. Proc Natl Acad Sci USA 94 4312-4317 (1997).
Kroetz, D.L., Yook, P., Costet, P., et al. Peroxisome proliferator-activated receptor α controls the hepatic CYP4A induction adaptive response to starvation and diabetes. J Biol Chem 273 31581-31589 (1998).
Martin, G., Schoonjans, K., Lefevbre, A., et al. Coordinate regulation of the expression of the fatty acid transport protein and acyl-CoA synthetase genes by PPARα and PPARγ activators. J Biol Chem 272 28210-28217 (1997).
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| Size |
Global Purchasing |
| 25 µg |
|
| 50 µg |
|
| 100 µg |
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Description
Source:
recombinant His-tagged protein purified from E. coli
·
Mr:
~34 kDa
·
Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear receptor family of ligand activated transcription factors that heterodimerize with retinoic acid like receptor to regulate gene expression and differentiation.1 The PPAR family of nuclear hormone receptors consists of three subtypes encoded by separate genes: PPARα, PPARδ (also referred to as hNUC1, PPARβ, or FAAR), and PPARγ. Among them PPARα is highly expressed in tissues displaying a high catabolic rate of fatty acids such as the liver, skeletal muscles, brown fat, heart, kidneys, and cells of artherosclerotic lesions. 2 Fatty acids, NSAIDs, prostaglandins, leukotriene B4, WY 14643, and hypolipidemic drugs are PPARα ligands.3,4,5 PPARα transcriptionally regulates a variety of genes involved in fatty acid metabolism and oxidation, such as acyl-CoA oxidase, enol-CoA hydratase, medium chain fatty acyl-CoA dehydrogenase, fatty acid transport protein, and cytochrome P450 4A isozymes.6,7 Cayman’s PPARα LBD (amino acids 170-430 of full length human PPARα) has been expressed and purified from E. coli. The purity was determined using gel electrophoresis followed by coomassie staining and western blot analysis. Functionality of PPARα LBD was tested using a fluorescent ligand binding assay.
1
Kersten, S., Desvergne, B., and Wahli, W. Roles of PPARs in health and disease. Nature 405 421-424 (2000).
2
Berger, J., Leibowitz, M.D., Doebber, T.W., et al. Novel peroxisome proliferator-activated receptor (PPAR) γ and PPARδ ligands produce distinct biological effects. J Biol Chem 274 6718-6725 (1999).
3
Devchand, P.R., Keller, H., Peters, J.M., et al. The PPARα-leukotriene B4 pathway to inflammation control. Nature 384 39-43 (1996).
4
Lehmann, J.M., Lenhard, J.M., Oliver, B.B., et al. Peroxisome proliferator-activated receptors a and g are activated by indomethacin and other non-steroidal anti-inflammatory drugs. J Biol Chem 272 3406-3410 (1997).
5
Forman, B.M., Chen, J., and Evans, R.M. Hypolipidemic drugs, polyunsaturated fatty acids, and eicosanoids are ligands for peroxisome proliferator-activated receptors α and δ. Proc Natl Acad Sci USA 94 4312-4317 (1997).
6
Martin, G., Schoonjans, K., Lefevbre, A., et al. Coordinate regulation of the expression of the fatty acid transport protein and acyl-CoA synthetase genes by PPARα and PPARγ activators. J Biol Chem 272 28210-28217 (1997).
7
Kroetz, D.L., Yook, P., Costet, P., et al. Peroxisome proliferator-activated receptor α controls the hepatic CYP4A induction adaptive response to starvation and diabetes. J Biol Chem 273 31581-31589 (1998).
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