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Cat. Number
702159255809020
Chemical Name
PPARα Transcription Factor Assay Kit
References
Synonyms
  • Peroxisome Proliferator-activated Receptor α Transcription Factor Assay Kit
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Olefsky, J.M. Nuclear receptor minireview series. J Biol Chem 276(40) 36863-36864 (2001).

Park, K.S., Ciaraldi, T.P., Lindgren, K., et al. Troglitazone effects on gene expression in human skeletal muscle of type II diabetes involve up-regulation of peroxisome proliferator-activated receptor-γ. J Clin Endocrinol Metab 83 2830-2835 (1998).

Willson, T.M., Brown, P.J., Sternbach, D.D., et al. The PPARs: From orphan receptors to drug discovery. J Med Chem 43(4) 528-550 (2000).

Gervois, P., Torra, I.P., Fruchart, J., et al. Regulation of lipid and lipoprotein metabolism by PPAR activators. Clin Chem Lab Med 38(1) 3-11 (2000).

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10006915-96WELL
Transcription Factor Binding Assay Buffer (4X) 1 ea
Transcription Factor Antibody Binding Buffer (10X) 1 ea
Transcription Factor Goat Anti-Rabbit HRP Conjugate 1 ea
Transcription Factor PPAR Competitor dsDNA 1 ea
Transcription Factor PPAR 96-Well Strip Plate 1 ea
Transcription Factor Developing Solution 1 ea
Transcription Factor Stop Solution 1 ea
Transcription Factor PPARα Positive Control 1 ea
Transcription Factor PPARα Primary Antibody 1 ea
Transcription Factor Reagent A 1 ea
Polysorbate 20 1 ea
Wash Buffer Concentrate (400X) 5 mL
Size Global Purchasing
96 wells  

Description

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPARα, β/δ, and γ, which are expressed at variable levels in different tissues.1 The Cayman Chemical PPARα Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPARα binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE and PPARα is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.

1 Olefsky, J.M. Nuclear receptor minireview series. J Biol Chem 276(40) 36863-36864 (2001).