| References |
| Synonyms |
- Peroxisome Proliferator-activated Receptor γ Full Length (human recombinant from Sf21 cells)
|
| Formulation |
A solution in 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride, 1 mM DTT, and 20% glycerol |
| Purity |
≥80% by SDS-PAGE |
| Stability |
6 months |
| Storage |
-80°C |
| Shipping |
Dry ice
in continental US; may vary elsewhere
|
Background Reading
Kersten, S., Desvergne, B., and Wahli, W. Roles of PPARs in health and disease. Nature 405 421-424 (2000).
Clark, R.B. The role of PPARs in inflammation and immunity. J Leukoc Biol 71 388-400 (2002).
Vidal-Puig, A., Jimenez-Linan, M., Lowell, B.B., et al. Regulation of PPAR γ gene expression by nutrition and obesity in rodents. J Clin Invest 97 2553-2561 (1996).
| Size |
Global Purchasing |
| 5 µg |
|
| 10 µg |
|
| 25 µg |
|
| 50 µg |
|
Description
Source:
human recombinant N-terminal His-tagged protein expressed in Sf21 insect cells
·
Mr:
~60 kDa
·
Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear receptor family of ligand activated transcription factors that heterodimerizes with retinoic acid-like receptor to regulate gene expression and differentiation.1 The PPAR family of nuclear hormone receptors consists of three subtypes encoded by separate genes: PPARα, PPARδ, and PPARγ Among them PPARγ is the most widely studied and has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer.2 PPARγ is primarily expressed in adipose tissue and to a lesser extent in colon, immune system, and the retina.3 Cayman’s PPARγ full length (FL) protein is expressed and purified from Sf21 insect cells. The purity was determined using gel electrophoresis followed by coomassie staining. Ligand binding functionality was confirmed using a fluorescent-based ligand binding assay. The functionality of the DNA binding domain (DBD) was tested using Cayman’s PPARγ Transcription Factor Assay Kit (Catalog No. 10006855).
1
Kersten, S., Desvergne, B., and Wahli, W. Roles of PPARs in health and disease. Nature 405 421-424 (2000).
2
Vidal-Puig, A., Jimenez-Linan, M., Lowell, B.B., et al. Regulation of PPAR γ gene expression by nutrition and obesity in rodents. J Clin Invest 97 2553-2561 (1996).
3
Clark, R.B. The role of PPARs in inflammation and immunity. J Leukoc Biol 71 388-400 (2002).
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