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Cat. Number
702096745195402
Chemical Name
PPARγ Polyclonal Antibody
References
Synonyms
  • Peroxisome Proliferator-activated Receptor γ

FormulationPeptide affinity-purified IgG in TBS, containing 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide
Stability1 year
Storage-20°C
ShippingWet icein continental US; may vary elsewhere
Specificity
Human PPARγ1 and PPARγ2+
Murine PPARγ1 and PPARγ2+

Background Reading

Greene, M.E., Blumberg, B., McBride, O.W., et al. Isolation of the human peroxisome proliferator activated receptor γ cDNA: Expression in hematopoietic cells and chromosomal mapping. Gene Expr 4 281-299 (1995).

Fajas, L., Auboeu, D., Raspé, E., et al. The organization, promoter analysis, and expression of the human PPARγ gene. J Biol Chem 272 18779-18789 (1997).

Elbrecht, A., Chen, Y., Cullinan, C.A., et al. Molecular cloning, expression and characterization of human peroxisome proliferator activated receptors γ 1 and γ 2. Biochem Biophys Res Commun 224 431-437 (1996).

Thuillier, P., Baillie, R., Sha, X., et al. Cytosolic and nuclear distribution of PPARγ2 in differentiating 3T3-L1 preadipocytes. J Lipid Res 39 2329-2338 (1998).

Spiegelman, B.M., and Flier, J.S. Adipogenesis and obesity: Rounding out the big picture. Cell 87 377-389 (1995).

Lemberger, T., Desvergne, B., and Wahli, W. Peroxisome proliferator-activated receptors: A nuclear receptor signaling pathway in lipid physiology. Annu Rev Cell Dev Biol 12 335-363 (1996).

Mukherjee, R., Jow, L., Croston, G.E., et al. Identification, characterization, and tissue distribution of human peroxisome proliferator-activated receptor (PPAR) isoforms PPARγ2 versus PPARγ1 and activation with retinoid X receptor agonists and antagonists. J Biol Chem 272 8071-8076 (1997).

Show all 7Hide all but first 3

SizeGlobal Purchasing
500 µl 

Description

Antigen:human PPARγ1 amino acids 82-101 (PASPPYYSEKTQLYNKPHEE; amino acids 110-129 of PPARγ2)·Host:rabbit·Application(s):WB·PPARγ is a ligand-activated transcription factor involved in the regulation of lipid homeostasis and may function as a master regulator of adipogenesis.1,2,3,4 PPARγ mRNA is expressed prominently in adipose tissue, but is also found in large intestine, kidney, liver, and small intestine.5 Alternative splicing of the PPARγ gene results in at least two mRNA species that differ at their 5' ends.1,6 Human PPARγ1 and PPARγ2 proteins are 53 and 57 kDa, respectively, based on the deduced amino acid sequences.6 PPARγ2 is the major PPARγ isoform found in both the cytosolic and nuclear fractions of undifferentiated 3T3-L1 cells.7 Total cellular PPARγ2 protein increases approximately 2-fold following differentiation of 3T3-L1 cells, whereas only small quantities of PPARγ1 are detected in the nuclear fraction following differentiation.7

1Greene, M.E., Blumberg, B., McBride, O.W., et al. Isolation of the human peroxisome proliferator activated receptor γ cDNA: Expression in hematopoietic cells and chromosomal mapping. Gene Expr 4 281-299 (1995).

2Elbrecht, A., Chen, Y., Cullinan, C.A., et al. Molecular cloning, expression and characterization of human peroxisome proliferator activated receptors γ 1 and γ 2. Biochem Biophys Res Commun 224 431-437 (1996).

3Spiegelman, B.M., and Flier, J.S. Adipogenesis and obesity: Rounding out the big picture. Cell 87 377-389 (1995).

4Lemberger, T., Desvergne, B., and Wahli, W. Peroxisome proliferator-activated receptors: A nuclear receptor signaling pathway in lipid physiology. Annu Rev Cell Dev Biol 12 335-363 (1996).

5Fajas, L., Auboeu, D., Raspé, E., et al. The organization, promoter analysis, and expression of the human PPARγ gene. J Biol Chem 272 18779-18789 (1997).

6Mukherjee, R., Jow, L., Croston, G.E., et al. Identification, characterization, and tissue distribution of human peroxisome proliferator-activated receptor (PPAR) isoforms PPARγ2 versus PPARγ1 and activation with retinoid X receptor agonists and antagonists. J Biol Chem 272 8071-8076 (1997).

7Thuillier, P., Baillie, R., Sha, X., et al. Cytosolic and nuclear distribution of PPARγ2 in differentiating 3T3-L1 preadipocytes. J Lipid Res 39 2329-2338 (1998).

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