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Cat. Number
702091666921073
Chemical Name
PPARγ Transcription Factor Assay Kit
References
Synonyms
  • Peroxisome Proliferator-activated Receptor γ Transcription Factor Assay Kit
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Olefsky, J.M. Nuclear receptor minireview series. J Biol Chem 276(40) 36863-36864 (2001).

Kersten, S., Desvergne, B., and Wahli, W. Roles of PPARs in health and disease. Nature 405 421-424 (2000).

Sakamoto, J., Kimura, H., Moriyama, S., et al. Activation of human peroxisome proliferator-activated receptor (PPAR) subtypes by pioglitazone. Biochem Biophys Res Commun 278 704-711 (2000).

Usui, S., Suzuki, T., Hattori, Y., et al. Design, synthesis, and biological activity of novel PPARγ ligands based on rosiglitazone and 15d-PGJ2. Bioorg Med Chem Lett 15 1547-1551 (2005).

Vidal-Puig, A., Jimenez-Linan, M., Lowell, B.B., et al. Regulation of PPAR γ gene expression by nutrition and obesity in rodents. J Clin Invest 97 2553-2561 (1996).

Clark, R.B. The role of PPARs in inflammation and immunity. J Leukoc Biol 71 388-400 (2002).

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10006855-96WELL
Transcription Factor Binding Assay Buffer (4X) 1 ea
Transcription Factor PPARγ Positive Control 1 ea
Transcription Factor Antibody Binding Buffer (10X) 1 ea
Transcription Factor PPARγ Primary Antibody 1 ea
Transcription Factor Goat Anti-Rabbit HRP Conjugate 1 ea
Transcription Factor PPAR Competitor dsDNA 1 ea
Transcription Factor PPAR 96-Well Strip Plate 1 ea
Transcription Factor Developing Solution 1 ea
Transcription Factor Stop Solution 1 ea
Transcription Factor Reagent A 1 ea
Polysorbate 20 1 ea
Wash Buffer Concentrate (400X) 5 mL
Size Global Purchasing
96 wells  

Description

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPARα, β/δ, and γ, which are expressed at variable levels in different tissues.1 The Cayman Chemical PPARγ Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPARγ binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE and PPARγ is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.

1 Olefsky, J.M. Nuclear receptor minireview series. J Biol Chem 276(40) 36863-36864 (2001).