| References |
| Synonyms |
- Hypoxia Inducible Factor-1α
|
| Formulation |
Peptide affinity-purified IgG |
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
|
| Specificity |
| Human HIF-1α |
+ |
| Murine HIF-1α |
+ |
| Simian HIF-1α |
+ |
|
Background Reading
Wenger, R.H. Cellular adaptation to hypoxia: O2-sensing protein hydroxylases, hypoxia-inducible transcription factors, and O2 regulated gene expression. FASEB J 16 1151-1162 (2002).
Ambrosin, G., Nath, A.K., Sierra-Honigmann, M.R., et al. Transcriptional activation of the human leptin gene in response to hypoxia. Involvement of hypoxia-inducible factor 1.. J Biol Chem 277 34601-34609 (2002).
Liu, X.H., Kirschenbaum, A., Lu, M., et al. Prostaglandin E2 induces hypoxia-inducible factor-1α stabilization and nuclear localization in a human prostate cancer cell line. J Biol Chem 277(51) 50081-50086 (2002).
Lando, D., Peet, D.J., Whelan, D.A., et al. Asparagine hydroxylation of the HIF transactivation domain: a hypoxic switch. Science 295 858-861 (2002).
Richard, D.E., Berra, E., Gothié, E., et al. p42/p44 Mitogen-activated protein kinases phosphorylate hypoxia-inducible factor 1α (HIF-1α) and enhance the transcriptional activity of HIF-1. J Biol Chem 274(46) 32631-32637 (1999).
Blancher, C., Moore, J.W., Talks, K.L., et al. Relationship of hypoxia-inducible factor (HIF)-1α and HIF-2α expression to vascular endothelial growth factor induction and hypoxia survival in human breast cancer cell lines. Cancer Res 60 7106-7113 (2000).
Wang, G.L., Jiang, B., Rue, E.A., et al. Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci USA 92 5510-5514 (1995).
Talks, K.L., Turley, H., Gatter, K.C., et al. The expression and distribution of the hypoxia-inducible factors HIF-1α and HIF-2α in normal human tissues, cancers, and tumor-associated macrophages. Am J Pathol 157(2) 411-421 (2000).
Koukourakis, M.I., Giatromanolaki, A., Skarlatos, J., et al. Hypoxia inducible factor (HIF-1α and HIF-2α) expression in early esophageal cancer and response to photodynamic therapy and radiotherapy. Cancer Res 61 1830-1832 (2001).
Show all 9
Hide all but first 3
| Size |
Global Purchasing |
| 500 µl |
|
Description
Antigen:
HIF-1α amino acids 809-826
·
Host:
rabbit
·
Application(s):
(+) WB; (−) ICC and IP; other applications not tested
·
Hypoxia inducible factor-1α (HIF-1α) is a transcription factor that accumulates under low-oxygen conditions.1,2 Following hypoxic stimulus and cytoplasmic accumulation, HIF-1α migrates to the nucleus where with other transcription factors, it drives the production of stress-adaptive proteins. This response is essential for maintenance of normal oxidative physiology, however overexpression in cancer cells promotes tumor survival.2,3,4,5,6 HIF-1α is an endothelial PAS domain protein 1 (EPAS-1) family member with phosphorylation dependent activities.7,8 Cayman Chemical’s HIF-1α polyclonal antibody can be used for WB analysis for HIF-1α on samples from human, mouse, and simian. It is not suitable for ICC or IP. Other applications for use of this antibody have not yet been tested.
1
Wenger, R.H. Cellular adaptation to hypoxia: O2-sensing protein hydroxylases, hypoxia-inducible transcription factors, and O2 regulated gene expression. FASEB J 16 1151-1162 (2002).
2
Wang, G.L., Jiang, B., Rue, E.A., et al. Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci USA 92 5510-5514 (1995).
3
Talks, K.L., Turley, H., Gatter, K.C., et al. The expression and distribution of the hypoxia-inducible factors HIF-1α and HIF-2α in normal human tissues, cancers, and tumor-associated macrophages. Am J Pathol 157(2) 411-421 (2000).
4
Koukourakis, M.I., Giatromanolaki, A., Skarlatos, J., et al. Hypoxia inducible factor (HIF-1α and HIF-2α) expression in early esophageal cancer and response to photodynamic therapy and radiotherapy. Cancer Res 61 1830-1832 (2001).
5
Blancher, C., Moore, J.W., Talks, K.L., et al. Relationship of hypoxia-inducible factor (HIF)-1α and HIF-2α expression to vascular endothelial growth factor induction and hypoxia survival in human breast cancer cell lines. Cancer Res 60 7106-7113 (2000).
6
Ambrosin, G., Nath, A.K., Sierra-Honigmann, M.R., et al. Transcriptional activation of the human leptin gene in response to hypoxia. Involvement of hypoxia-inducible factor 1.. J Biol Chem 277 34601-34609 (2002).
7
Richard, D.E., Berra, E., Gothié, E., et al. p42/p44 Mitogen-activated protein kinases phosphorylate hypoxia-inducible factor 1α (HIF-1α) and enhance the transcriptional activity of HIF-1. J Biol Chem 274(46) 32631-32637 (1999).
8
Liu, X.H., Kirschenbaum, A., Lu, M., et al. Prostaglandin E2 induces hypoxia-inducible factor-1α stabilization and nuclear localization in a human prostate cancer cell line. J Biol Chem 277(51) 50081-50086 (2002).
|
