Antigen:
human HIF-1α amino acids 432-528
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Clone designation:
H1α67
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Host:
mouse
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Application(s):
IHC, WB
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Hypoxia inducible factor-1α (HIF-1α) is a transcription factor that accumulates under low-oxygen conditions.1,2 Following hypoxic stimulus and cytoplasmic accumulation, HIF-1α migrates to the nucleus where, with other transcription factors, it drives the production of stress-adaptive proteins. This response is essential for maintenance of normal oxidative physiology, however overexpression in cancer cells promotes tumor survival.1,3,4,5,6 HIF-1α is an EPAS-1 family member with phosphorylation dependent activities. HIF-1α will migrate to the apparent molecular weights of 110-125 kDa (by SDS-PAGE) depending on phosphorylation status.7,8 Positive controls include human, primate, and murine cell cultures grown in hypoxic conditions or treated with prolyl hydroxylase inhibitors such as DMOG (Item No. 71210), 2,4-DPD (Item No. 71200), desferrioxamine, or CoCl2.9,6
1
Wang, G.L., Jiang, B., Rue, E.A., et al. Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci USA 92 5510-5514 (1995).
2
Wenger, R.H. Cellular adaptation to hypoxia: O2-sensing protein hydroxylases, hypoxia-inducible transcription factors, and O2 regulated gene expression. FASEB J 16 1151-1162 (2002).
3
Talks, K.L., Turley, H., Gatter, K.C., et al. The expression and distribution of the hypoxia-inducible factors HIF-1α and HIF-2α in normal human tissues, cancers, and tumor-associated macrophages. Am J Pathol 157(2) 411-421 (2000).
4
Koukourakis, M.I., Giatromanolaki, A., Skarlatos, J., et al. Hypoxia inducible factor (HIF-1α and HIF-2α) expression in early esophageal cancer and response to photodynamic therapy and radiotherapy. Cancer Res 61 1830-1832 (2001).
5
Blancher, C., Moore, J.W., Talks, K.L., et al. Relationship of hypoxia-inducible factor (HIF)-1α and HIF-2α expression to vascular endothelial growth factor induction and hypoxia survival in human breast cancer cell lines. Cancer Res 60 7106-7113 (2000).
6
Ambrosin, G., Nath, A.K., Sierra-Honigmann, M.R., et al. Transcriptional activation of the human leptin gene in response to hypoxia. Involvement of hypoxia-inducible factor 1.. J Biol Chem 277 34601-34609 (2002).
7
Richard, D.E., Berra, E., Gothié, E., et al. p42/p44 Mitogen-activated protein kinases phosphorylate hypoxia-inducible factor 1α (HIF-1α) and enhance the transcriptional activity of HIF-1. J Biol Chem 274(46) 32631-32637 (1999).
8
Liu, X.H., Kirschenbaum, A., Lu, M., et al. Prostaglandin E2 induces hypoxia-inducible factor-1α stabilization and nuclear localization in a human prostate cancer cell line. J Biol Chem 277(51) 50081-50086 (2002).
9
Lando, D., Peet, D.J., Whelan, D.A., et al. Asparagine hydroxylation of the HIF transactivation domain: a hypoxic switch. Science 295 858-861 (2002).
