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Cat. Number
070208626500089
Chemical Name
iNOS Electrophoresis Standard
References
Synonyms
  • NOS II
  • Inducible Nitric Oxide Synthase
Formulation 5 µg purified iNOS in 50 µl 50 mM HEPES, pH 7.5, containing 20% glycerol
Purity >95%
Stability 1 year
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Hevel, J.M., White, K.A., and Marletta, M.A. Purification of the inducible murine macrophage nitric oxide synthase. Identification as a flavoprotein. J Biol Chem 266 22789-22791 (1991).

Balligand, J., Ungureanu-Longrois, D., Simmons, W.W., et al. Cytokine-inducible nitric oxide synthase (iNOS) expression in cardiac myocytes. Characterization and regulation of iNOS expression and detection of iNOS activity in single cardiac cyocytes in vivo. J Biol Chem 269 27580-27588 (1994).

Schmidt, H.H.H.W., Warner, T.D., Nakane, M., et al. Regulation and subcellular location of nitrogen oxide synthases in RAW264.7 macrophages. Mol Pharmacol 41 615-624 (1992).

Ogura, T., and Esumi, H. Nitric oxide synthase expression in human neuroblastoma cell line induced by cytokines. Mol Neurosci 7 853-856 (1996).

Knowles, R.G., and Moncada, S. Nitric oxide synthases in mammals. Biochem J 298 249-258 (1994).

Lorsbach, R.B., Murphy, W.J., Lowenstein, C.J., et al. Expression of the nitric oxide synthase gene in mouse macrophages activated for tumor cell killing. Molecular basis for the synergy between interferon-γ and lipopolysaccharide. J Biol Chem 268 1908-1913 (1993).

Michel, T., Xie, Q., and Nathan, C. Molecular biological analysis of nitric oxide synthases. 161-175 (1996).

Walker, G., Pfeilschifter, J., and Kunz, D. Mechanism of suppression of inducible nitric-oxide synthase (iNOS) expression in interferon (IFN)-γ-stimulated RAW 264.7 cells by dexamethasone. Evidence for glucocorticoid-induced degradation of iNOS protein by calpain as a key step in post-transcriptional regulation. J Biol Chem 271 16679-16687 (1996).

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Size Global Purchasing
5 µg  

Description

Mr: 130.00 · Each vial contains 5 µg of iNOS (murine macrophage) to be used as a standard for WB and electrophoresis. The enzyme has been carefully purified to exclude all unrelated proteins; may not be catalytically active.1

1 Hevel, J.M., White, K.A., and Marletta, M.A. Purification of the inducible murine macrophage nitric oxide synthase. Identification as a flavoprotein. J Biol Chem 266 22789-22791 (1991).

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