| References |
| Synonyms |
- Calcium-independent Phospholipase A2
|
| Formulation |
Lyophilized antibody |
| Stability |
2 years |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
|
Background Reading
Ackermann, E.J., Kempner, E.S., and Dennis, E.A. Ca2+-independent cytosolic phospholipase A2 from macrophage-like P388D1 cells. J Biol Chem 269 9227-9233 (1994).
Wolf, M.J., and Gross, R.W. Expression, purification, and kinetic characterization of a recombinant 80-kDa intracellular calcium-independent phospholipase A2. J Biol Chem 271 30879-30885 (1996).
Balboa, M.A., Balsinde, J., Jones, S.S., et al. Identity between the Ca2+-independent phospholipase A2 enzymes from P388D1 macrophages and Chinese hamster ovary cells. J Biol Chem 272 8576-8580 (1997).
Wolf, M.J., and Gross, R.W. The calcium-dependent association and functional coupling of calmodulin with myocardial phospholipase A2. Implications for cardiac cycle-dependent alterations in phospholipolysis. J Biol Chem 271 20989-20992 (1996).
Wolf, M.J., Wang, J., Turk, J., et al. Depletion of intracellular calcium stores activates smooth muscle cell calcium-independent phospholipase A2. J Biol Chem 272 1522-1526 (1997).
Dennis, E.A. The growing phospholipase A2 superfamily of signal transduction enzymes. Trends Biochem Sci 22 1-2 (1997).
Tang, J., Kriz, R.W., Wolfman, N., et al. A novel cytosolic calcium-independent phospholipase A2 contains eight ankyrin motifs. J Biol Chem 272 8567-8575 (1997).
Atsumi, G., Murakami, M., Kojima, K., et al. Distinct roles of two intracellular phospholipase A2s in fatty acid release in the cell death pathway. Proteolytic fragment of type IVA cytosolic phospholipase A2α inhibits stimulus-induced arachidonate release, whereas that of type VI Ca2+-independent phospholipase A2 augments spontaneous fatty acid release. J Biol Chem 275 18248-18258 (2000).
Isenovic, E., and LaPointe, M.C. Role of Ca2+-independent phospholipase A2 in the regulation of inducible nitric oxide synthase in cardiac myocytes. Hypertension 35 249-254 (2000).
Roshak, A.K., Capper, E.A., Stevenson, C., et al. Human calcium-independent phospholipase A2 mediates lymphocyte proliferation. J Biol Chem 275 35692-35698 (2000).
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| Size |
Global Purchasing |
| 1 ea |
|
Description
Antigen:
hamster iPLA2 amino acids 557-576 (PRFNQNINLKPPTQPADQLV)
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Host:
rabbit
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Application(s):
WB; other applications not tested
·
Calcium-independent phospholipase A2 (iPLA2) is one of the most recently characterized isoforms in the growing list of the phospholipase A2 enzyme family.1 iPLA2 has been purified or cloned from CHO cells and mouse P388D1 macrophage-like cells and is the major isoform of phospholipase A2 found in A-10 smooth muscle cells.2,3,4,5,6,7 The enzyme has a molecular weight of about 80-85 kDa and is classified as a type VI PLA2.1-5 The enzyme from CHO cells is approximately 95% and 90% homologous to mouse and human iPLA2, respectively.2,3
1
Dennis, E.A. The growing phospholipase A2 superfamily of signal transduction enzymes. Trends Biochem Sci 22 1-2 (1997).
2
Tang, J., Kriz, R.W., Wolfman, N., et al. A novel cytosolic calcium-independent phospholipase A2 contains eight ankyrin motifs. J Biol Chem 272 8567-8575 (1997).
3
Balboa, M.A., Balsinde, J., Jones, S.S., et al. Identity between the Ca2+-independent phospholipase A2 enzymes from P388D1 macrophages and Chinese hamster ovary cells. J Biol Chem 272 8576-8580 (1997).
4
Wolf, M.J., and Gross, R.W. Expression, purification, and kinetic characterization of a recombinant 80-kDa intracellular calcium-independent phospholipase A2. J Biol Chem 271 30879-30885 (1996).
5
Ackermann, E.J., Kempner, E.S., and Dennis, E.A. Ca2+-independent cytosolic phospholipase A2 from macrophage-like P388D1 cells. J Biol Chem 269 9227-9233 (1994).
6
Wolf, M.J., Wang, J., Turk, J., et al. Depletion of intracellular calcium stores activates smooth muscle cell calcium-independent phospholipase A2. J Biol Chem 272 1522-1526 (1997).
7
Wolf, M.J., and Gross, R.W. The calcium-dependent association and functional coupling of calmodulin with myocardial phospholipase A2. Implications for cardiac cycle-dependent alterations in phospholipolysis. J Biol Chem 271 20989-20992 (1996).
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