Antigen:
synthetic peptide corresponding to human IRAK-1 amino acids 700-712
·
Host:
rabbit
·
Application(s):
IP and WB
·
Peptide sequence:
amino acids 700-712
·
Nuclear factor-κB (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNF-α, and LPS. A serine/threonine protein kinase associated with interleukin-1 receptor-associated kinase 1 (IRAK-1) and its homologue murine pelle-like protein kinase (mPLK) has been identified.1,2 IRAK-1 is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response.3 IRAK-1 mediates a signaling cascade leading to NF-κB activation by members of the IL-1 family including IL-1 and IL-18 (also known as IGIF).4,2
1
Huang, J., Gao, X., Li, S., et al. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein. Proc Natl Acad Sci USA 34.1 12829-12832 (1997).
2
Robinson, D., Shibuya, K., Mui, A., et al. IGIF does not drive Th1 development but synergizes with IL-12 for interform-g production and activated IRAK and NFkB. Immunity 7 571-581 (1997).
3
Trofimova, M., Sprenkle, A.B., Green, M., et al. Developmental and tissue-specific expression of mouse pelle-like protein kinase. J Biol Chem 271(30) 17609-17612 (1996).
4
Cao, Z., Henzel, W.J., and Gao, X. IRAK: A kinase associated with the interleukin-1 receptor. Science 271 1128-1131 (1996).
