Antigen:
synthetic peptide corresponding to a mixture of mouse IRAK-4 amino acids 38-54 and 120-136
·
Host:
rabbit
·
Application(s):
IP and WB
·
Specific activity:
amino acids 38-54 and 120-136
·
The toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an interleukin-1 (IL-1) receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules.1 Interleukin-1 receptor-associated kinases (IRAKs) are important mediators in the signal transduction of Toll/IL-1 receptor (TIR) family members.2 The cytoplasmic domains of TIR proteins interact with the adapter protein, MyD88. MyD88 then recruits IRAKs (IRAK-1-4), which in turn interact with other adapter molecules, such as TRAF6 to activate NF-κB and MAPK pathways. A member of this family, IRAK-4 has been identified.3 IRAK-4 may act as an upstream activator of IRAK-1. IRAK-4 is important for LPS activation of TLRs. Mice lacking IRAK4 are resistant to lethal doses of LPS and are also severtly impaired in their responses to viral and bacterial challenges.4,5
1
Muzio, M., Natoli, G., Saccani, S., et al. The human toll signaling pathway: Divergence of nuclear factor κB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6). J Exp Med 187(12) 2097-2101 (1998).
2
Cao, Z., Xiong, J., Takeuchi, M., et al. TRAF6 is a signal transducer for interleukin-1. Nature 383 443-446 (1996).
3
Li, S., Strelow, A., Fontana, E.J., et al. IRAK-4: A novel member of the IRAK family with the properties of an IRAK-kinase. Proc Natl Acad Sci USA 99(8) 5567-5572 (2002).
4
Suzuki, N., Suzuki, S., Duncan, G.S., et al. Severe impairment of interleukin-1 and toll-like receptor signalling in mice lacking IRAK-4. Nature 416 750-756 (2002).
5
Yeh, W. IRAK-4 as the central TIR signaling mediator in innate immunity. Trends Immunol 23(10) 503-506 (2002).
