IκBα (cleavage specific) Monoclonal Antibody (Clone 5D1623)_上海惠诚生物生物试剂,标准品,仪器设备,耗材,一站式服务

服务热线：13917250134「同微信」

您的位置：首页>>Cayman>>Antibodies>>IκBα (cleavage specific) Monoclonal Antibody (Clone 5D1623)

产品中心

/ Products Classification 点击展开+

Cat. Number

070130895206412

Chemical Name

IκBα (cleavage specific) Monoclonal Antibody (Clone 5D1623)

References

Formulation

100 µg of protein G-purified IgG in 200 µl PBS, containing 0.05% BSA and 0.05% sodium azide.

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Human IκBα

Size	Global Purchasing
1 ea

Description

Antigen: synthetic peptide corresponding to the caspase-3 mediated cleavage site of IκBα · Clone designation: 5D1623 · Host: mouse · Isotype: IgG₁ · Application(s): WB · NF-κB is silenced in the cytoplasm by an inhibitory protein IκB.¹ Synthesis of IκBα is autoregulated.² IκB proteins are phosphorylated by IκB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β, and IKK3/γ.^3,4,5,6 External stimuli such as tumor necrosis factor or other cytokines induce phosphorylation and degradation of IκB releasing NF-κB dimers. NF-κB dimers subsequently translocate to the nucleus and activate target genes. Six members of the IκB family have been identified.¹ IκBa can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IκBa (ΔN-IκBα).^7,8 The ΔN-IκBα is resistant to degradation in response to inducers of NF-κB and act as a dominant inhibitory molecule that suppresses NF-κB activity during apoptosis.

1 Verma, I.M., Stevenson, J.K., Schwarz, E.M., et al. Rel/NF-κB/IκB family: Intimate tales of association and dissociation. Genes Dev 9 2723-2735 (1995).

2 Verma, I.M., and Stevenson, J. IκB kinase: Beginning, not the end. Proc Natl Acad Sci USA 94 11758-11760 (1997).

3 DiDonato, J.A., Hayakawa, M., Rothwarf, D.M., et al. A cytokine-responsive IκB kinase that activates the transcription factor NF-κB. Nature 388 548-554 (1997).

4 Régnier, C.H., Song, H.Y., Gao, X., et al. Identification and characterization of an IκB kinase. Cell 90 373-383 (1997).

5 Mercurio, F., Zhu, H., Murray, B.W., et al. IKK-1 and IKK-2: Cytokine-activated IκB kinases essential for NF-κB activation. Science 278(5339) 860-866 (1997).

6 Yamaoka, S., Courtois, G., Bessia, C., et al. Complementation cloning of NEMO, a component of the IκB kinase complex essential for NF-κB activation. Cell 93 1231-1240 (1998).

7 Barkett, M., Xue, D., Horvitz, H.R., et al. Phosphorylation of IκNα inhibits its cleavage by caspase CPP32 in vitro. J Biol Chem 272(47) 29419-29422 (1997 Nov 21 1997).

8 Reuther, J.Y., and Baldwin, A.S. Apoptosis promotes a caspase-induced amino-terminal truncation of IκBα that functions as a stable inhibitor of NF-κB. J Biol Chem 274 20664-20670 (1999).

下一个：IRAK-4 Polyclonal Antibody上一个：IκBα Monoclonal Antibody - biotin (Clone 6A920)

联系我们

电话：021-60498804
邮箱：info@e-biochem.com
地址：上海市徐汇区漕溪路258弄27号航星商务楼2-404

扫一扫，关注我们

惠诚品牌

产品中心: 分子生物; 分析化学; 生化试剂; 仪器耗材

企业动态

技术资料

在线咨询联系方式二维码

服务热线

021-60498804

扫一扫，关注我们