| References |
| Synonyms |
- MAP Kinase Kinase 1
- MAPKK1
|
| Formulation |
Affinity-purified IgG |
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
|
| Specificity |
| Human MEK1 |
+ |
| Rat MEK1 |
+ |
| Bovine MEK1 (expected) |
+ |
| Canine MEK1 (expected) |
+ |
| Chicken MEK1 (expected) |
+ |
| Murine MEK1 (expected) |
+ |
| Non-human primates ME 1 (expected) |
+ |
| Xenopus MEK1 (expected) |
+ |
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|
Background Reading
Crews, C.M., Alessandrini, A., and Erikson, R.L. The primary structure of MEK a protein kinase that phosphorylates the ERK gene product (extracellular signal-regulated kinases). Science 258 478-480 (1992).
Seger, R., Ahn, N.G., Boulton, T.G., et al. Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: Implications for their mechanism of activation.. Proc Natl Acad Sci USA 88 6142-6146 (1991).
Kyriakis, J.M., Brautigan, D.L., Ingebritsen, T.S., et al. pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity.. J Biol Chem 266(16) 10043-10046 (1991).
Mansour, S.J., Resing, K.A., Candi, J.M., et al. Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: Determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis. J Biochem 116 304-314 (1994).
Adams, J.P., and Sweatt, J.D. Molecular psychology: Roles for the ERK MAP kinase cascade in memory.. Annu Rev Pharmacol Toxicol 42 135-163 (2002).
Chong, H., Vikis, H.G., and Guan, K.L. Mechanisms of regulating the Raf kinase family.. Cell Signal 15 463-469 (2003).
Ahn, N.G. The MAP kinase cascade. Discovery of a new signal transduction pathway.. Mol Cell Biochem 127-128 201-209 (1993).
Show all 7
Hide all but first 3
| Size |
Global Purchasing |
| 1 ea |
|
Description
Antigen:
phosphopeptide corresponding to amino acid residues surrounding the Phospho-Thr292 of human MEK1
·
Host:
rabbit
·
Application(s):
WB
·
MAP kinase kinase 1 (MAPKK1) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation 1,2. This pathway also plays a key role in synaptic plasticity in the brain 3. Activated MEK1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase 4,5,6. Conversely, there also appears to be a feedback phosphorylation of MEK1 by MAP kinase. The sites on MEK1 that are phosphorylated by MAP kinase on Thr292 and Thr386 7.
1
Ahn, N.G. The MAP kinase cascade. Discovery of a new signal transduction pathway.. Mol Cell Biochem 127-128 201-209 (1993).
2
Chong, H., Vikis, H.G., and Guan, K.L. Mechanisms of regulating the Raf kinase family.. Cell Signal 15 463-469 (2003).
3
Adams, J.P., and Sweatt, J.D. Molecular psychology: Roles for the ERK MAP kinase cascade in memory.. Annu Rev Pharmacol Toxicol 42 135-163 (2002).
4
Kyriakis, J.M., Brautigan, D.L., Ingebritsen, T.S., et al. pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity.. J Biol Chem 266(16) 10043-10046 (1991).
5
Seger, R., Ahn, N.G., Boulton, T.G., et al. Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: Implications for their mechanism of activation.. Proc Natl Acad Sci USA 88 6142-6146 (1991).
6
Crews, C.M., Alessandrini, A., and Erikson, R.L. The primary structure of MEK a protein kinase that phosphorylates the ERK gene product (extracellular signal-regulated kinases). Science 258 478-480 (1992).
7
Mansour, S.J., Resing, K.A., Candi, J.M., et al. Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: Determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis. J Biochem 116 304-314 (1994).
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