| References |
| Synonyms |
|
| Formulation |
200 µg peptide affinity-purified IgG in 500 µl TBS, pH 7.4, containing 50% glycerol, 0.1% BSA, and 0.02% sodium azide |
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
|
| Specificity |
| Human MC4R |
+ |
| Murine MC4R |
+ |
| Rat MC4R |
+ |
|
Background Reading
Yeo, G.S.H., and Siddle, K. Attractin' more attention-new pieces in the obesity puzzle? Biochem J 376 e7-e8 (2003).
Gantz, I., Miwa, H., Konda, Y., et al. Molecular cloning, expression, and gene localization of a fourth melanocortin receptor. J Biol Chem 268(20) 15174-15179 (1993).
Crowley, V.E.F., Yeo, G.S.H., and O'Rahilly, S. Obesity therapy: Altering the energy intake-and-expenditure balance sheet. Nat Rev Drug Discov 1 276-286 (2002).
Ho, G., and MacKenzie, R.G. Functional characterization of mutations in melanocortin-4 receptor associated with human obesity. J Biol Chem 274(50) 35816-35822 (1999).
Fan, W., Boston, B.A., Kesterson, R.A., et al. Role of melanocortinergic neurons in feeding and the agouti obesity syndrome. Nature 385 165-168 (1997).
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| Size |
Global Purchasing |
| 500 µl |
|
Description
Antigen:
mouse melanocortin-4 receptor amino acids 21-33 (YGLHSNASESLGK)
·
Host:
rabbit
·
Application(s):
WB and IHC (formalin-fixed paraffin-embedded sections); other applications not tested
·
Melanocortins are known to exert a broad array of physiological actions including 'melanogenesis, steroidogenesis, sexual function and inflammation, as well as appetite regulation and energy homeostasis'.1 These actions are mediated (differentially or concurrently) in part by a family of G-protein coupled receptors, the melanocortin receptors 1-5 (MC1R-MC5R). Melanocortin-4 receptor (MC4R) was first cloned in 1993 with transcripts expressed primarily in the brain.2 Genetic studies of mice and humans have established a critical role of MC4R in appetite regulation.3 Heterozygous mutations in MC4R account for 1-6% of severe cases of human obesity. Thus, MC4R has been a prime target for therapeutic intervention in obesity.4 All known mammalian MC4Rs are 332 amino acids in length with an estimated molecular weight of 37 kDa. The protein is heavily glycosylated and appears at multiple positions on SDS-PAGE.5 Cayman’s MC4R polyclonal antibody mainly detects an unglycosylated protein at 37 kDa and a glycosylated protein at 55 kDa.
1
Yeo, G.S.H., and Siddle, K. Attractin' more attention-new pieces in the obesity puzzle? Biochem J 376 e7-e8 (2003).
2
Gantz, I., Miwa, H., Konda, Y., et al. Molecular cloning, expression, and gene localization of a fourth melanocortin receptor. J Biol Chem 268(20) 15174-15179 (1993).
3
Fan, W., Boston, B.A., Kesterson, R.A., et al. Role of melanocortinergic neurons in feeding and the agouti obesity syndrome. Nature 385 165-168 (1997).
4
Crowley, V.E.F., Yeo, G.S.H., and O'Rahilly, S. Obesity therapy: Altering the energy intake-and-expenditure balance sheet. Nat Rev Drug Discov 1 276-286 (2002).
5
Ho, G., and MacKenzie, R.G. Functional characterization of mutations in melanocortin-4 receptor associated with human obesity. J Biol Chem 274(50) 35816-35822 (1999).
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