References |
Synonyms |
|
Formulation |
Peptide affinity-purified IgG |
Stability |
1 year |
Storage |
-20°C |
Shipping |
Wet ice
in continental US; may vary elsewhere
|
Specificity |
Human monoglyceride lipase |
+ |
Murine monoglyceride lipase |
+ |
Bovine monoglyceride lipase |
+ |
Rat monoglyceride lipase |
+ |
Show all 4
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|
Background Reading
Dinh, T.P., Freund, T.F., and Piomelli, D. A role for monoglyceride lipase in 2-arachidonoylglycerol inactivation. Chem Phys Lipids 121 149-158 (2002).
Dinh, T.P., Carpenter, D., Leslie, F.M., et al. Brain monoglyceride lipase participating in endocannabinoid inactivation. Proc Natl Acad Sci USA 99(16) 10819-10824 (2002).
Karlsson, M., Contreras, J.A., Hellman, U., et al. cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases. J Biol Chem 272 27218-27223 (1997).
Size |
Global Purchasing |
500 µl |
|
Description
Antigen:
human monoacylglycerol lipase amino acids 1-14
·
Host:
rabbit
·
Application(s):
IHC and WB
·
Endocannabinoids, such as arachidonoyl ethanolamide (AEA) and 2-Arachidonoyl glycerol (2-AG), function as short-range modulators of cell and synaptic activity. Monoacylglycerol lipase (MAGL) hydrolyzes 2-AG to terminate its biological actions 1 and works consecutively with hormone-sensitive lipase (HSL) to mobilize fatty acids from the triglyceride stores of adipocytes.2 MAGL has a molecular weight of ~33 kDa and exhibits a high degree of homology among human, mouse, and rat at the amino acid level.3,4,1,2 The enzyme is expressed in a variety of tissues with most abundant expression in skeletal muscle and adipose tissue, suggesting a role of MAGL in monoglyceride hydrolysis in diverse tissues. Cayman Chemical’s MAGL polyclonal antibody can be used for western blot and immunohistochemical (formalin-fixed paraffin-embedded tissue) analysis for MAGL on samples of human, mouse, rat, and bovine origin. Other applications for use of this antibody have not yet been tested.
1
Dinh, T.P., Carpenter, D., Leslie, F.M., et al. Brain monoglyceride lipase participating in endocannabinoid inactivation. Proc Natl Acad Sci USA 99(16) 10819-10824 (2002).
2
Karlsson, M., Reue, K., Xia, Y., et al. Exon-intron organization and chromosomal localization of the mouse monoglyceride lipase gene. Gene 272 11-18 (2001).
3
Karlsson, M., Contreras, J.A., Hellman, U., et al. cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases. J Biol Chem 272 27218-27223 (1997).
4
Dinh, T.P., Freund, T.F., and Piomelli, D. A role for monoglyceride lipase in 2-arachidonoylglycerol inactivation. Chem Phys Lipids 121 149-158 (2002).
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