| References |
| Formulation |
200 µg of peptide in 200 µl of TBS, pH 7.4, containing 0.1% BSA and 0.02% sodium azide |
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
|
Background Reading
Okamoto, Y., Morishita, J., Tsuboi, K., et al. Molecular characterization of a phospholipase D generating anandamide and its congeners. J Biol Chem 279(7) 5298-5305 (2004).
Moesgaard, B., Petersen, G., Jaroszewski, J.W., et al. Age dependent accumulation of N-acyl-ethanolamine phospholipids in ischemic rat brain: A 31P NMR and enzyme activity study. J Lipid Res 41 985-990 (2000 Jan 1).
Hansen, H.S., Moesgaard, B., Petersen, G., et al. Putative neuroprotective actions of N-acyl-ethanolamines. Pharmacol Ther 95 119-126 (2002).
| Size |
Global Purchasing |
| 200 µg |
|
Description
To be used in conjunction with Cayman’s NAPE-PLD polyclonal antibody (aa 6-20) (Item No. 10306) to block protein-antibody complex formation during analysis of NAPE-PLD · N-acylethanolamines (NAEs) are involved in diverse biological processes such as inflammatory regulation, apoptosis, and tissue degeneration.1 In animals, NAEs are mainly biosynthesized via a membrane phospholipid-dependent pathway, which is the enzymatic hydrolysis of N-acyl-phosphatidylethanolamine (NAPE). The enzyme catalyzing this reaction is a phospholipase D subtype selective for NAPE named N-acylphosphatidylethanolamine-hydrolysing phospholipase D (NAPE-PLD). It has been cloned from mouse, rat, and human and is 393-396 amino acids in length, with an estimated molecular weight of 46 kDa. Both NAPE-PLD mRNA and protein activity have been detected in a wide range of tissues with the highest levels in brain, kidney, and testis.2 In rat, NAPE-PLD activity in the brain is low in neonates and is 15-fold higher in adults, whereas the activity remains constant in the heart during development.3
1
Hansen, H.S., Moesgaard, B., Petersen, G., et al. Putative neuroprotective actions of N-acyl-ethanolamines. Pharmacol Ther 95 119-126 (2002).
2
Okamoto, Y., Morishita, J., Tsuboi, K., et al. Molecular characterization of a phospholipase D generating anandamide and its congeners. J Biol Chem 279(7) 5298-5305 (2004).
3
Moesgaard, B., Petersen, G., Jaroszewski, J.W., et al. Age dependent accumulation of N-acyl-ethanolamine phospholipids in ischemic rat brain: A 31P NMR and enzyme activity study. J Lipid Res 41 985-990 (2000 Jan 1).
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