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Cat. Number

069668415967205

Chemical Name

Nitrotyrosine Monoclonal Antibody - Biotinylated

References

Formulation

Peptide affinity-purified IgG

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Species independent detection of nitrotyrosine	+
Chlorotyrosine	<5%

Background Reading

Beckman, J.S., Chen, J., Ischiropoulos, H., et al. Oxidative chemistry of peroxynitrite. Methods Enzymol 233 229-240 (1994).

Wang, P., and Zweier, J.L. Measurement of nitric oxide and peroxynitrite generation in the postischemic heart. Evidence for peroxynitrite-mediated reperfusion injury. J Biol Chem 271 29223-29230 (1996).

Beckman, J.S., and Koppenol, W.H. Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and the ugly. Am J Physiol 271 C1424-C1437 (1996).

Beckman, J.S., Ye, Y.Z., Anderson, P.G., et al. Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry. Biol Chem Hoppe-Seyler 375 81-88 (1994).

Myatt, L., Rosenfield, R.B., Eis, A.L.W., et al. Nitrotyrosine residues in placenta. Evidence of peroxynitrite formation and action. Hypertension 28 488-493 (1996).

Chou, S.M., Wang, H.S., and Komai, K. Colocalization of NOS and SOD1 in neurofilament accumulation within motor neurons of amyotrophic lateral sclerosis: An immunohistochemical study. J Chem Neuroanat 10 249-258 (1996).

Franze, T., Weller, M.G., Niessner, R., et al. Comparison of nitrotyrosine antibodies and development of immunoassays for the detection of nitrated proteins. Analyst 129 589-596 (2004).

Ferrante, R.J., Shinobu, L.A., Schulz, J.B., et al. Increased 3-nitrotyrosine and oxidative damage in mice with a human copper/zinc superoxide dismutase mutation. Ann Neurol 42 326-334 (1997).

Abe, K., Pan, L., Watanabe, M., et al. Upregulation of protein-tyrosine nitration in the anterior horn cells of amyotrophic lateral sclerosis. Neurol Res 19 124-128 (1997).

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Size	Global Purchasing
100 µg

Description

Antigen: peroxynitrite-treated KLH · Host: mouse · Application(s): WB, IHC, IP, enzyme-linked immunosorbent assay; other applications not tested · Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by NO synthase. NO reacts rapidly with superoxide (6.7 x 109 M⁻¹sec⁻¹) to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo.^1,2 Cayman Chemical’s biotinylated nitrotyrosine monoclonal antibody can be used for the species-independent detection of nitrotyrosine using a variety of immunochemical techniques. Biotinylation of the antibody allows for detection using avidin-enzyme or avidin-fluorophore conjugates and provides approximately 2-fold better sensitivity compared to the unbiotinylated antibody (Catalog No. 189542).

1 Beckman, J.S., Chen, J., Ischiropoulos, H., et al. Oxidative chemistry of peroxynitrite. Methods Enzymol 233 229-240 (1994).

2 Beckman, J.S., and Koppenol, W.H. Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and the ugly. Am J Physiol 271 C1424-C1437 (1996).

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