| References |
| Synonyms |
- Platelet-activating Factor Receptor
|
| Formulation |
Peptide affinity-purified IgG |
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
|
| Specificity |
| Human PAF receptor |
+ |
| Mouse PAF receptor |
+ |
| Porcine PAF receptor |
+ |
| Rat PAF receptor |
+ |
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Background Reading
Mutoh, H., Bito, H., Minami, M., et al. Two different promoters direct expression of two distinct forms of mRNAs of human platelet-activating factor receptor. FEBS Lett 322 129-134 (1993).
Quellet, S., Müller, E., and Rola-Pleszczynski, M. IFN-γ up-regulates platelet-activating factor receptor gene expression in human monocytes. J Immunol 152 5092-5099 (1994).
Nakamura, M., Honda, Z., Izumi, T., et al. Molecular cloning and expression of platelet-activating factor receptor from human leukocytes. J Biol Chem 266(30) 20400-20405 (1991).
Marrache, A.M., Gobeil, F. Jr., Bernier, S.G., et al. Proinflammatory gene induction by platelet-activating factor mediated via its cognate nuclear receptor. J Immunol 169(11) 6474-6481 (2002).
Ye, R.D., Prossnitz, E.R., Zou, A., et al. Characterization of a human cDNA that encodes a functional receptor for platelet activating factor. Biochem Biophys Res Commun 180 105-111 (1991).
Kunz, D., Gerard, N.P., and Gerard, C. The human leukocyte platelet-activating factor receptor. cDNA cloning, cell surface expression, and construction of a novel epitope-bearing analog. J Biol Chem 267 9101-9106 (1992).
Bito, H., Honda, Z., Nakamura, M., et al. Cloning, expression and tissue distribution of rat platelet-activating-factor-receptor cDNA. Eur J Biochem 227 211-218 (1994).
Müller, E., Dagenais, P., Alami, N., et al. Identification and functional characterization of platelet-activating factor receptors in human leukocyte populations using polyclonal anti-peptide antibody. Proc Natl Acad Sci USA 90 5818-5822 (1993).
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| Size |
Global Purchasing |
| 500 µl |
|
Description
Antigen:
human PAF receptor amino acids 1-17 (MEPHDSSHMDSEFRYTL)
·
Host:
rabbit
·
Application(s):
FC, ICC, and WB
·
PAF is a potent phospholipid mediator which exerts diverse biological actions by interaction with a G protein-coupled PAF receptor. The PAF receptor has been cloned from a number of species including human, rat, and guinea pig and is characterized as a 7-transmembrane receptor which induces phosphoinositol turnover through G-protein coupling.1,2,3,4,5 Northern blot analysis reveals that the receptor is expressed in leukocytes, placenta, lung, spleen, small intestine, kidney, liver, and brain.3,4 In leukocyte cell populations the receptor is found on platelets, monocytes, neutrophils, and B-cells, whereas resting T-cells and natural killer cell lines do not express the PAF receptor.6 Human monocytes treated with INF-γ have a 2-6 fold increase in PAF receptor expression compared to untreated cells.7 PAF receptor is detected on immunoblot at 48 kDa.8
1
Nakamura, M., Honda, Z., Izumi, T., et al. Molecular cloning and expression of platelet-activating factor receptor from human leukocytes. J Biol Chem 266(30) 20400-20405 (1991).
2
Kunz, D., Gerard, N.P., and Gerard, C. The human leukocyte platelet-activating factor receptor. cDNA cloning, cell surface expression, and construction of a novel epitope-bearing analog. J Biol Chem 267 9101-9106 (1992).
3
Ye, R.D., Prossnitz, E.R., Zou, A., et al. Characterization of a human cDNA that encodes a functional receptor for platelet activating factor. Biochem Biophys Res Commun 180 105-111 (1991).
4
Bito, H., Honda, Z., Nakamura, M., et al. Cloning, expression and tissue distribution of rat platelet-activating-factor-receptor cDNA. Eur J Biochem 227 211-218 (1994).
5
Mutoh, H., Bito, H., Minami, M., et al. Two different promoters direct expression of two distinct forms of mRNAs of human platelet-activating factor receptor. FEBS Lett 322 129-134 (1993).
6
Müller, E., Dagenais, P., Alami, N., et al. Identification and functional characterization of platelet-activating factor receptors in human leukocyte populations using polyclonal anti-peptide antibody. Proc Natl Acad Sci USA 90 5818-5822 (1993).
7
Quellet, S., Müller, E., and Rola-Pleszczynski, M. IFN-γ up-regulates platelet-activating factor receptor gene expression in human monocytes. J Immunol 152 5092-5099 (1994).
8
Marrache, A.M., Gobeil, F. Jr., Bernier, S.G., et al. Proinflammatory gene induction by platelet-activating factor mediated via its cognate nuclear receptor. J Immunol 169(11) 6474-6481 (2002).
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