| References |
| Formulation |
Peptide affinity-purified antibody |
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
|
| Specificity |
| Human progesterone receptor |
+ |
| Murine progesterone receptor (expected) |
+ |
| Non-human primate progesterone receptor (expected) |
+ |
| Rat progesterone receptor |
+ |
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Background Reading
Edwards, D.P. Regulation of signal transduction pathways by estrogen and progesterone. Annu Rev Physiol 67 335-376 (2005).
Attia, G.R., Zeitoun, K., Edwards, D., et al. Progesterone receptor isoform A but not B is expressed in endometriosis. J Clin Endocrinol Metab 85 2897-2902 (2000).
Clemm, D.L., Sherman, L., Boonyaratanakornkit, V., et al. Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody. Mol Endocrinol 14 52-65 (2000).
Lin, V.C.L., Woon, C.T., Aw, S.E., et al. Distinct molecular pathways mediate progesterone-induced growth inhibition and focal adhesion. Endocrinology 144 5650-5657 (2003).
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| Size |
Global Purchasing |
| 1 ea |
|
Description
Antigen:
phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser294 of human progesterone receptor
·
Host:
mouse
·
Application(s):
WB and IHC (frozen sections)
·
There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer.1 The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones.2,3 Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor.4 Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.
1
Edwards, D.P. Regulation of signal transduction pathways by estrogen and progesterone. Annu Rev Physiol 67 335-376 (2005).
2
Attia, G.R., Zeitoun, K., Edwards, D., et al. Progesterone receptor isoform A but not B is expressed in endometriosis. J Clin Endocrinol Metab 85 2897-2902 (2000).
3
Lin, V.C.L., Woon, C.T., Aw, S.E., et al. Distinct molecular pathways mediate progesterone-induced growth inhibition and focal adhesion. Endocrinology 144 5650-5657 (2003).
4
Clemm, D.L., Sherman, L., Boonyaratanakornkit, V., et al. Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody. Mol Endocrinol 14 52-65 (2000).
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