| References |
| Synonyms |
- PDCD4
- Neoplastic Transformation Inhibitor
|
| Formulation |
Peptide-affinity purified IgG polyclonal IgG in 500 µl TBS, pH 7.4, containing 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide |
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
|
| Specificity |
| Human PDCD4 |
+ |
| Murine PDCD4 |
+ |
| Rat PDCD4 |
+ |
|
Background Reading
Nieves-Alicea, R., Colburn, N.H., Simeone, A., et al. Programmed cell death 4 inhibits breast cancer cell invasion by increasing tissue inhibitor of metalloproteinases-2 expression. Breast Cancer Res Treat (2008).
Cmarik, J.L., Min, H., Hegamyer, G., et al. Differentially expressed protein Pdcd4 inhibits tumor promoter-induced neoplastic transformation. Proc Natl Acad Sci USA 96(24) 14037-14042 (1999).
Bitomsky, N., Wethkamp, N., Marikkannu, R., et al. siRNA-mediated knockdown of Pdcd4 expression causes upregulation of p21(Waf1/Cip1) expression. Oncogene (2008).
Yang, H., Cho, M., Zakowicz, H., et al. A novel function of the MA-3 domains in transformation and translation suppressor Pdcd4 is essential for its binding to eukaryotic translation initiation factor 4A. Mol Cell Biol 24(9) 3894-3906 (2004).
Böhm, M., Sawicka, K., Siebrasse, J.P., et al. The transformation suppressor protein Pdcd4 shuttles between nucleus and cytoplasm and binds RNA. Oncogene 22 4905-4910 (2003).
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| Size |
Global Purchasing |
| 500 µl |
|
Description
Antigen:
human PDCD4 C-terminal amino acids 458-468 (GDGGRLKPESY)
·
Host:
rabbit
·
Application(s):
WB
·
Programmed cell death protein 4 (PDCD4) levels are elevated during apoptosis and absent in many cancer samples.1,2 Loss of PDCD4 expression is an important event in cancer cell progression whereas the restoration of PDCD4 protein can lower metalloproteinase activity and possible metastasis.2,3 The known isoforms of PDCD4 differ only at the N-terminial with isoform 1 comprised of 468 amino acids and isoform 2 comprised of 458 amino acids. Each isoform contains two MA-3 domains essential for binding of PDCD4 binding to eIF4A, thereby suppressing cell cycle regulation and growth factor production.3,2,4 This antibody is capable of detecting both PDCD4 isoforms. Additional protein modifications are possible and can explain the range of masses detected by immunoblotting (52-64 kDa).1 The molecular mechanisms of PDCD4 influence on tumor suppression are becoming known but warrant further research.3,5,2
1
Cmarik, J.L., Min, H., Hegamyer, G., et al. Differentially expressed protein Pdcd4 inhibits tumor promoter-induced neoplastic transformation. Proc Natl Acad Sci USA 96(24) 14037-14042 (1999).
2
Nieves-Alicea, R., Colburn, N.H., Simeone, A., et al. Programmed cell death 4 inhibits breast cancer cell invasion by increasing tissue inhibitor of metalloproteinases-2 expression. Breast Cancer Res Treat (2008).
3
Bitomsky, N., Wethkamp, N., Marikkannu, R., et al. siRNA-mediated knockdown of Pdcd4 expression causes upregulation of p21(Waf1/Cip1) expression. Oncogene (2008).
4
Yang, H., Cho, M., Zakowicz, H., et al. A novel function of the MA-3 domains in transformation and translation suppressor Pdcd4 is essential for its binding to eukaryotic translation initiation factor 4A. Mol Cell Biol 24(9) 3894-3906 (2004).
5
Böhm, M., Sawicka, K., Siebrasse, J.P., et al. The transformation suppressor protein Pdcd4 shuttles between nucleus and cytoplasm and binds RNA. Oncogene 22 4905-4910 (2003).
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