Robert, A., Schultz, J.R., Nezamis, J.E., et al. Gastric antisecretory and antiulcer properties of PGE2, 15-methyl PGE2, and 16,16-dimethyl PGE2. Intravenous, oral and intrajejunal administration. Gastroenterology 70 359-370 (1976).
Graff, G., and Anderson, L.A. 1-(4-(3-(4-(bis(4-fluorophenyl)hydroxymethyl)-1-piperidinyl) propoxy)-3-methoxyphenyl)ethanone (AHR-5333): A selective human blood neutrophil 5-lipoxygenase inhibitor. Prostaglandins 38 473-496 (1989).
Arvind, P., Papavassiliou, E.D., Tsioulias, G.J., et al. Prostaglandin E2 down-regulates the expression of HLA-DR antigen in human colon adenocarcinoma cell lines. Biochemistry 34 5604-5609 (1995).
Willis, A.L., and Cornelsen, M. Repeated injection of prostaglandin E2 in rat paws induces chronic swelling and a marked decrease in pain threshold. Prostaglandins 3 353-357 (1973).
Tanikawa, N., Ohmiya, Y., Ohkubo, H., et al. Identification and characterization of a novel type of membrane-associated prostaglandin E synthase. Biochem Biophys Res Commun 291(4) 884-889 (2002).
Jackson, G.M., Sharp, H.T., and Varner, M.W. Cervical ripening before induction of labor: A randomized trial of prostaglandin E2 gel versus low-dose oxytocin. Am J Obstet Gynecol 171 1092-1096 (1994).
Watanabe, K., Kurihara, K., and Suzuki, T. Purification and characterization of membrane-bound prostaglandin E synthase from bovine heart. Biochim Biophys Acta 1439 406-414 (1999).
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Antigen:
synthetic peptide from the human mPGEs-2 sequence conjugated to KLH (amino acids 221-235; VYGGKEARTEEMKWR)
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Host:
rabbit
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Application(s):
WB
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Prostaglandin E synthase-2 (microsomal) (mPGES-2) was recently identified as a second membrane-associated PGE2 producing enzyme.1,2 Unlike mPGES-1, mPGES-2 does not require glutathione for catalytic activity and is activated in the presence of a broad range of reducing agents. Northern blotting suggests this enzyme is found in high quantity in heart, brain, skeletal muscle, and kidney. Cayman Chemical’s mPGES-2 polyclonal antibody can be used for Western blot analysis for mPGES-2 on samples from a variety of species including human, mouse, sheep, cow, and monkey. Use 1:1,000 dilution for formalin fixed paraffin embedded tissues. The antibody does not react with mPGES-1 or cytosolic PGES. Other applications for use of this antibody have not yet been tested.
1
Watanabe, K., Kurihara, K., and Suzuki, T. Purification and characterization of membrane-bound prostaglandin E synthase from bovine heart. Biochim Biophys Acta 1439 406-414 (1999).
2
Tanikawa, N., Ohmiya, Y., Ohkubo, H., et al. Identification and characterization of a novel type of membrane-associated prostaglandin E synthase. Biochem Biophys Res Commun 291(4) 884-889 (2002).
