| References |
| Synonyms |
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| Formulation |
Peptide affinity-purified IgG in TBS containing 50% glycerol, 0.1% BSIgG, lyophilized |
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
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Background Reading
Jakobsson, P., Thorén, S., Morgenstern, R., et al. Identification of human prostaglandin E synthase: A microsomal, glutathione-dependent, inducible enzyme, constituting a potential novel drug target. Proc Natl Acad Sci USA 96 7220-7225 (1999).
Tanioka, T., Nakatani, Y., Semmyo, N., et al. Molecular identification of cytosolic prostaglandin E2 synthase that is functionally coupled with cyclooxygenase-1 immediate prostaglandin E2 biosynthesis. J Biol Chem 275 32775-32782 (2000).
| Size |
Global Purchasing |
| 1 ea |
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Description
Antigen:
human cPGE synthase amino acids 58-67 (CIDPNDSKHK)
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Host:
rabbit
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Application(s):
WB and IHC
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Prostaglandin E Synthase (PGE Synthase) catalyzes the isomerization of PGH2 to PGE2. Cytosolic and microsomal PGE synthase enzymes have been cloned and characterized. Cytosolic PGE synthase (cPGE synthase) is a glutathione-dependent enzyme with a predicted size of 18.6 kDa (23 kDa on SDS-PAGE). The enzyme is expressed in a wide variety of tissues and cells, the levels of which are unaffected by treatment with IL-1β and TNFα.1 However, enzyme expression increases approximately 3-fold in rat brain following LPS treatment.1 Microsomal PGE synthase is a 16 kDa protein expressed in a variety of tissues including prostate, testes, and small intestine, as well as in A549 and HeLa cells.2 In contrast to cPGE synthase, mPGE synthase protein expression is increased in A549 cells following treatment with IL-1β.2 The two enzymes show <10% homology at the amino acid level.
1
Tanioka, T., Nakatani, Y., Semmyo, N., et al. Molecular identification of cytosolic prostaglandin E2 synthase that is functionally coupled with cyclooxygenase-1 immediate prostaglandin E2 biosynthesis. J Biol Chem 275 32775-32782 (2000).
2
Jakobsson, P., Thorén, S., Morgenstern, R., et al. Identification of human prostaglandin E synthase: A microsomal, glutathione-dependent, inducible enzyme, constituting a potential novel drug target. Proc Natl Acad Sci USA 96 7220-7225 (1999).
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