| References |
| Formulation |
Affinity-purified IgG |
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
|
| Specificity |
| (+)Rat Raf-1 |
+ |
| Bovine Raf-1 (expected) |
+ |
| Canine Raf-1 (expected) |
+ |
| Chicken Raf-1 (expected) |
+ |
| Human Raf-1 (expected) |
+ |
| Murine Raf-1 (expected) |
+ |
| Non-human primate Raf-1 (expected) |
+ |
| Xenopus Raf-1 (expected) |
+ |
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|
Background Reading
Dhillon, A.S., and Kolch, W. Untying the regulation of the Raf-1 kinase. Arch Biochem Biophys 404 3-9 (2002).
Dougherty, M.K., Müller, J., Ritt, D.A., et al. Regulation of Raf-1 by direct feedback phosphorylation. Mol Cell 17 215-224 (2005).
Chong, H., Vikis, H.G., and Guan, K.L. Mechanisms of regulating the Raf kinase family.. Cell Signal 15 463-469 (2003).
Marshall, C.J. Ras effectors. Curr Opin Cell Biol 8 197-204 (1996).
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| Size |
Global Purchasing |
| 1 ea |
|
Description
Antigen:
phosphopeptide corresponding to amino acid residues surrounding phospho-Ser301 of rat raf-1
·
Host:
rabbit
·
Application(s):
WB
·
The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK.1 Previous studies have shown that phosphorylation is required for Raf-1 activation.2,3 Recent work has demonstrated that phosphorylation also regulates the down regulation of Raf with two sites participating: Ser301 and Ser642.4
1
Marshall, C.J. Ras effectors. Curr Opin Cell Biol 8 197-204 (1996).
2
Chong, H., Vikis, H.G., and Guan, K.L. Mechanisms of regulating the Raf kinase family.. Cell Signal 15 463-469 (2003).
3
Dhillon, A.S., and Kolch, W. Untying the regulation of the Raf-1 kinase. Arch Biochem Biophys 404 3-9 (2002).
4
Dougherty, M.K., Müller, J., Ritt, D.A., et al. Regulation of Raf-1 by direct feedback phosphorylation. Mol Cell 17 215-224 (2005).
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