| References |
| Synonyms |
- EDG-1
- S1PR1
- Sphingosine-1-phosphate Receptor 1
|
| Formulation |
Peptide affinity-purified IgG |
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
|
Background Reading
Takuwa, Y., Takuwa, N., and Sugimoto, N. The Edg family G protein-coupled receptors for lysophospholipids: Their signaling properties and biological activities. J Biochem 131 767-771 (2002).
Kluk, M.J., and Hla, T. Signaling of sphingosine-1-phosphate via the S1P/EDG-family of G-protein-coupled receptors. Biochim Biophys Acta 1582 72-80 (2002).
Kohno, T., Wada, A., and Igarashi, Y. N-glycans of sphingosine 1-phosphate receptor Edg-1 regulate ligand-induced receptor internalization. FASEB J 16 983-992 (2002).
Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu Rev Biochem 73 321-354 (2004).
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| Size |
Global Purchasing |
| Trial Sufficient for 3 western blots |
|
| 500 µl |
|
Description
Antigen:
human S1P1 amino acids 241-253 (ISKASRSSEKSLA)
·
Host:
rabbit
·
Application(s):
WB, IHC (formalin-fixed paraffin-embedded sections), and ICC; other applications not tested
·
Sphingosine-1-phosphate (S1P) exerts its activity by binding to five distinct G-protein-coupled receptors, S1P1/EDG-1, S1P2/EDG-5, S1P3/EDG-3, S1P4/EDG-6, and S1P5/EDG-8.1,2 S1P1 primarily couples with pertussis toxin-sensitive Gi/o proteins to mediate S1P-induced cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis.1,2,3 Expression of S1P1 is abundant in embryological vasculature and is ubiquitously expressed in adult cells suggesting diverse physiological functions of this receptor.2 The human and murine S1P1 receptors have 382 amino acids with an estimated molecular weight of 43 kDa. Glycosylation at the N-terminal extracellular domain may cause the protein to migrate at a higher position in SDS-PAGE.4 Cayman’s S1P1 polyclonal antibody detects the receptor at 47 kDa by western blot analysis. The antibody can also be used for immunocytochemistry and immunohistochemistry to study expression patterns of this protein.
1
Takuwa, Y., Takuwa, N., and Sugimoto, N. The Edg family G protein-coupled receptors for lysophospholipids: Their signaling properties and biological activities. J Biochem 131 767-771 (2002).
2
Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu Rev Biochem 73 321-354 (2004).
3
Kluk, M.J., and Hla, T. Signaling of sphingosine-1-phosphate via the S1P/EDG-family of G-protein-coupled receptors. Biochim Biophys Acta 1582 72-80 (2002).
4
Kohno, T., Wada, A., and Igarashi, Y. N-glycans of sphingosine 1-phosphate receptor Edg-1 regulate ligand-induced receptor internalization. FASEB J 16 983-992 (2002).
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