| References |
| Synonyms |
- Sphingosine-1-phosphate Receptor 3
- S1PR3
- EDG-3
|
| Formulation |
Peptide affinity-purified IgG |
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
|
| Specificity |
| human S1P3 |
+ |
| murine S1P3 |
+ |
| rat S1P3 |
+ |
|
Background Reading
Takuwa, Y., Takuwa, N., and Sugimoto, N. The Edg family G protein-coupled receptors for lysophospholipids: Their signaling properties and biological activities. J Biochem 131 767-771 (2002).
Takuwa, Y. Subtype-specific differential regulation of Rho family G proteins and cell migration by the Edg family sphingosine-1-phosphate receptors. Biochem Biophys Acta 1582 112-120 (2002).
Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu Rev Biochem 73 321-354 (2004).
Kluk, M.J., and Hla, T. Signaling of sphingosine-1-phosphate via the S1P/EDG-family of G-protein-coupled receptors. Biochim Biophys Acta 1582 72-80 (2002).
Show all 4
Hide all but first 3
| Size |
Global Purchasing |
| 500 µl |
|
Description
Antigen:
human S1P3 amino acids 12-25 (VRGNETLREHYQYV)
·
Host:
rabbit
·
Application(s):
WB and ICC; other applications not tested
·
Sphingosine-1-phosphate (S1P) exerts its activity by binding to five distinct G-protein-coupled receptors, S1P1/EDG-1, S1P2/EDG-5, S1P3/EDG-3, S1P4/EDG-6, and S1P5/EDG-8.1,2 S1P3 couples to Gi/o-ERK, Gq-PLC, and G12/13-Rho axes to mediate S1P-induced cell proliferation, survival, migration, and related signaling events.1,2,3 S1P3 is widely expressed in various tissues, suggesting diverse physiological functions of this receptor.4 The human and murine S1P3 have 378 amino acids with an estimated molecular weight of 42 kDa. Glycosylation at the N-terminal extracellular domain may cause the protein to migrate at different positions in SDS-PAGE. Cayman’s S1P3 polyclonal antibody detects the receptor at 47 kDa using samples from human cerebral cortex. Liver and heart tissues display 2-3 bands between 40-50 kDa, possibly due to different degree of glycosylation.
1
Takuwa, Y., Takuwa, N., and Sugimoto, N. The Edg family G protein-coupled receptors for lysophospholipids: Their signaling properties and biological activities. J Biochem 131 767-771 (2002).
2
Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu Rev Biochem 73 321-354 (2004).
3
Kluk, M.J., and Hla, T. Signaling of sphingosine-1-phosphate via the S1P/EDG-family of G-protein-coupled receptors. Biochim Biophys Acta 1582 72-80 (2002).
4
Takuwa, Y. Subtype-specific differential regulation of Rho family G proteins and cell migration by the Edg family sphingosine-1-phosphate receptors. Biochem Biophys Acta 1582 112-120 (2002).
|
