Sphingosine Kinase 1 Polyclonal FITC Antibody_上海惠诚生物生物试剂,标准品,仪器设备,耗材,一站式服务

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Cat. Number

068976470664050

Chemical Name

Sphingosine Kinase 1 Polyclonal FITC Antibody

References

Synonyms

SPHK 1

Formulation

Fluorescein-conjugated affinity-purified IgG

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Murine SPHK 1	+
Human SPHK 1	+

Background Reading

Merrill, A.H., Sullards, M.C., Wang, E., et al. Sphingolipid metabolism: Roles in signal transduction and disruption by fumonisins. Environ Health Perspect 109(suppl. 2) 283-289 (2001).

Maceyka, M., Sankala, H., Hait, N.C., et al. SphK1 and SphK 2, sphingosine kinase isoenzymes with opposing functions in sphingolipid metabolism. J Biol Chem 280(44) 37118-37129 (2005).

Pitson, S.M., D'Andrea, R.J., Vandeleur, L., et al. Human sphingosine kinase: Purification, molecular cloning and characterization of the native and recombinant enzymes. Biochem J 350 429-441 (2000).

Kim, J., Kim, Y., Inagaki, Y., et al. Synthesis and evaluation of sphingoid analogs as inhibitors of sphingosine kinase. Bioorg Med Chem 13 3475-3485 (2005).

Delon, C., Manifava, M., Wood, E., et al. Sphingosine kinase 1 is an intracellular effector of phosphatidic acid. J Biol Chem 279(43) 44763-44774 (2004).

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Size	Global Purchasing
500 µl

Description

Antigen: human SPHK 1 amino acids 264-274 · Host: rabbit · Application(s): FC, IF, and WB · Peptide sequence: DLESEKYRRLG · The primary use of this antibody conjugate is for the detection of SPHK 1 in intact cells by direct immunolabeling methods such as flow cytometry or immunofluorescence microscopy. SPHK 1 is one of the enzymes involved in sphingolipid metabolism. SPHK 1 catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate. This reaction plays an important role in determining cell proliferation versus cell death.^1,2 SPHK 1 is found in a wide variety of tissues and cell types including kidney, liver, spleen, heart, platelets, and human tumors.³ On a cellular level, it is found in the cytosolic and membrane fractions.⁴ Based on the amino acid sequence, this protein has a molecular weight of approximately 43 kDa. Some reported post translational modifications may explain the shift in band migration to 50 kDa.⁵ NOTE: Multiple isoforms of SPHK 1 are known and one is 470 amino acids. This likely explains the 50 kDa band observed.

1 Delon, C., Manifava, M., Wood, E., et al. Sphingosine kinase 1 is an intracellular effector of phosphatidic acid. J Biol Chem 279(43) 44763-44774 (2004).

2 Merrill, A.H., Sullards, M.C., Wang, E., et al. Sphingolipid metabolism: Roles in signal transduction and disruption by fumonisins. Environ Health Perspect 109(suppl. 2) 283-289 (2001).

3 Kim, J., Kim, Y., Inagaki, Y., et al. Synthesis and evaluation of sphingoid analogs as inhibitors of sphingosine kinase. Bioorg Med Chem 13 3475-3485 (2005).

4 Maceyka, M., Sankala, H., Hait, N.C., et al. SphK1 and SphK 2, sphingosine kinase isoenzymes with opposing functions in sphingolipid metabolism. J Biol Chem 280(44) 37118-37129 (2005).

5 Pitson, S.M., D'Andrea, R.J., Vandeleur, L., et al. Human sphingosine kinase: Purification, molecular cloning and characterization of the native and recombinant enzymes. Biochem J 350 429-441 (2000).

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