Antigen:
fusion protein corresponding to amino acids 205-222 of human TRAF2
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Clone designation:
33A1293
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Host:
mouse
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Application(s):
WB
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Tumor necrosis factor (TNF)-induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor-associated factors (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-R superfamily (e.g., RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of seven TRAFs (TRAF1-TRAF7).1,2,3,4 TRAF2 is a 501-amino acid protein. Mutagenic studies suggest that the N-terminal RING finger and two adjacent zinc fingers of TRAF2 are required for NF-κB activation, whereas interaction with TNFR is mediated through the C-terminal domain.5,6 Distinct domains in the N- and C-termini are also required for association with TRAF1 and protein kinase receptor interacting protein (RIP). TRAF2 is involved in cellular resistance to TNF-induced apoptosis. TRAF2 deficient mice appear normal at birth; however, they die prematurely, probably due to atrophy of thymus, spleen, muscle mass and lack of TRAF2’s cytoprotective role.
1
Rothe, M., Wong, S.C., Henzel, W.J., et al. A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor. Cell 78(4) 681-92 (1994).
2
Cheng, G., Cleary, A.M., Ye, Z.S., et al. Involvement of CRAF1, a relative of TRAF, in CD40 signaling. Science 267(5203) 1494-8 (1995).
3
Nakano, H., Oshima, H., Chung, W., et al. TRAF5, an activator of NF-κB and putative signal transducer for the lymphotoxin-β receptor. J Biol Chem 271(25) 14661-4 (2011).
4
Cao, Z., Xiong, J., Takeuchi, M., et al. TRAF6 is a signal transducer for interleukin-1. Nature 383 443-446 (1996).
5
Takeuchi, M., Rothe, M., and Goeddel, D.V. Anatomy of TRAF2. Distinct domains for nuclear factor-κB activation and association with tumor necrosis factor signaling proteins.. J Biol Chem 271(33) 19935-42 (1996).
6
Park, Y.C., Burkitt, V., Villa, A.R., et al. Structural basis for self-association and receptor recognition of human TRAF2. Nature 398(6727) 533-8 (1999).
