Antigen:
fusion protein corresponding to amino acids 77-186 of human TRAF5
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Clone designation:
55A219
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Host:
mouse
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Application(s):
WB
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Tumor necrosis factor (TNF)-induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor-associated factors (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g., RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have led to identification of seven TRAFs (TRAF1-TRAF7).1,2,3,4,5 Human TRAF5 is a 557-amino acid protein. TRAF5 is implicated in NF-kB and c-Jun NH(2)-terminal kinase/stress-activated protein kinase activation by members of the TNF receptor superfamily, including CD27, CD30, CD40, and lymphotoxin-β receptor. Targeted disruption of TRAF5 gene causes defects in CD40-CD27 mediated lymphocyte activation.5
1
Nakano, H., Sakon, S., Koseki, H., et al. Targeted disruption of traf5 gene causes defects in CD40-and CD27-mediated lymphocyte activation. Proc Natl Acad Sci USA 96(17) 9803-8 (1999).
2
Cao, Z., Xiong, J., Takeuchi, M., et al. TRAF6 is a signal transducer for interleukin-1. Nature 383 443-446 (1996).
3
Rothe, M., Wong, S.C., Henzel, W.J., et al. A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor. Cell 78(4) 681-92 (1994).
4
Cheng, G., Cleary, A.M., Ye, Z.S., et al. Involvement of CRAF1, a relative of TRAF, in CD40 signaling. Science 267(5203) 1494-8 (1995).
5
Nakano, H., Oshima, H., Chung, W., et al. TRAF5, an activator of NF-κB and putative signal transducer for the lymphotoxin-β receptor. J Biol Chem 271(25) 14661-4 (2011).
