| Synonyms |
- P-gp Drug Interaction Assay Kit
|
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
|
| Size |
Global Purchasing |
| 96 wells |
|
Description
P-Glycoprotein (P-gp) is an active plasma membrane transporter involved in drug pharmacokinetics and cellular detoxification. P-gp exhibits a high drug-dependent ATP hydrolysis activity that is a reflection of its drug transport ability. The test of drug stimulation or inhibition of ATPase activity may be used to screen the potential drug interaction with P-gp. This assay provides an in vitro screening method for testing drug interaction with P-gp based on the study of modulation of basal or induced ATPase activity from enriched P-gp membrane vesicle preparations. P-gp ATPase activity is measured by a spectrophotometric method based on continuous monitoring of ADP formation in the vesicle suspension medium in a 96 well plate format. Data are analyzed by comparing the variation of the basal or stimulated activities to reference activities.
